欢迎访问《茶叶科学》,今天是

茶叶科学 ›› 2005, Vol. 25 ›› Issue (2): 100-108.doi: 10.13305/j.cnki.jts.2005.02.004

• • 上一篇    下一篇

儿茶素对ECV304细胞增殖和迁移的影响

张玉艳,沈生荣   

  1. 浙江大学茶叶科学研究所,浙江 杭州 310029
  • 出版日期:2005-06-25 发布日期:2019-09-16
  • 作者简介:张玉艳(1978— ),女,山东潍坊人,硕士研究生,主要从事茶叶生物化学和细胞生物学的研究。
  • 基金资助:
    国家自然科学基金资助项目(项目编号:30470198)

Effects of Catechins on the Proliferation and Migration of ECV304 Cell

ZHANG Yu-yan, SHEN Sheng-rong   

  1. Institute of Tea Sciences, Zhenjiang University, Hangzhou 310029, China
  • Online:2005-06-25 Published:2019-09-16

摘要: 利用体外细胞培养技术探讨了四种儿茶素对ECV304细胞增殖和迁移的影响。结果发现,EGCG对ECV304细胞存活率的影响最显著,在EGCG浓度为50~150 μmol/L时,细胞存活率达到极显著差异,半数抑制浓度IC50为75~150 μmol/L;EGCG对该细胞迁移作用影响明显,且呈剂量依赖性,IC50为150 μmol/L,EGCG可能通过抑制内皮细胞的迁移抑制新生血管的生成;ECG与ECV304作用36 h和48 h,IC50分别为390 μmol/L和370 μmol/L;C和EC与ECV304作用时,细胞存活率在80%以上。四种儿茶素在浓度低于75 μmol/L时,对正常ECV304细胞抑制影响不大。

关键词: 茶儿茶素, ECV304细胞, 细胞存活率, 细胞迁移

Abstract: By employing techniques of cell culture, the effects of four tea catechins, EGCG, ECG, EC and C were investigated on the proliferation and migration of ECV304 by the authors. The result demonstrated that EGCG was the most effective in cell viability among the four tested catechins. Viability of ECV304 were significantly different in the case of the concentrations of EGCG ,which was ranged from 50 to 150 μmol/L. The IC50 of EGCG was 75~150 μmol/L, EGCG was also effective in cell migration in a dose dependent manner, the IC50 was 150 μmol/L. EGCG could inhibit angiogensis, which may be related to the decreased migration of endothelial cell. The IC50 was at 390 μmol/L and 370 μmol/L in 36 hour-disposal and 48 hour-disposal of ECG respectively. The viability of ECV304 disposed by C and EC were above 80%. It was indicated that catechins showed insignificant inhibitory effect on normal cell growth and migration at the concentration of lower than 75 μmol/L.

Key words: Tea catechins, ECV304 cell, Cell viability, Cell migration

中图分类号: