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茶叶科学 ›› 2005, Vol. 25 ›› Issue (3): 172-176.doi: 10.13305/j.cnki.jts.2005.03.003

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茶树组培快繁技术的优化研究

周健, 成浩*, 王丽鸳   

  1. 农业部茶叶化学工程重点实验室,中国农业科学院茶叶研究所,浙江 杭州,310008
  • 出版日期:2005-09-25 发布日期:2019-09-16
  • 通讯作者: chenghao@mail.tricaas.com
  • 作者简介:周健(1979— )男,助理研究员,主要从事茶叶生物技术研究工作。
  • 基金资助:
    浙江省科技计划项目“茶树新品种快速繁育体系创新研究”资助项目,资助号:02402090

The Optimization Research on Tissue Culture and Rapid Propagation of Camellia Sinensis

ZHOU Jian, CHENG Hao*, WANG Li-yuan   

  1. Key Lab of Tea Chemical Engineering, Ministry of Agriculture;
    Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Online:2005-09-25 Published:2019-09-16

摘要: 本文试验分析了激素对茶树组培快繁中芽增殖和生长的影响,优化得到适宜的茶树组培苗增殖条件是:MS+BA 2.0+NAA 0.1+GA3 3.0βmg/L。该条件下培养30βd,茶树组培苗的增殖率可达2.75倍左右,高度大于5βcm的小苗成苗率在20%以上。

关键词: 茶树, 组织培养, 增殖, 成苗

Abstract: The effect of different hormone on proliferation and growth of tea tissue culture seedlings were investigated, and an optimal culture medium of MS + BA 2.0 + NAA 0.1 + GA3 3.0βmg/L was screened out. On this optimal medium, the proliferation rate of tea cultured buds was 2.75 times after 30’s incubation. And more than 20% of those buds could developed to seedlings higher than 5βcm.

Key words: Tea, Tissue culture, proliferation

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