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茶叶科学 ›› 2016, Vol. 36 ›› Issue (2): 111-118.doi: 10.13305/j.cnki.jts.2016.02.001

• •    下一篇

茶树β-葡萄糖苷酶研究进展

周汉琛, 雷攀登, 丁勇*   

  1. 安徽省农业科学院茶叶研究所,安徽 黄山 245000
  • 收稿日期:2015-08-13 出版日期:2016-04-15 发布日期:2019-08-23
  • 通讯作者: *,ahtdy@163.com
  • 作者简介:周汉琛,女,硕士研究生,主要从事茶叶生物化学方面的研究。
  • 基金资助:
    安徽省农业科学院院长基金项目(15B0837)、安徽省科技攻关计划(1501031100)、安徽省农业科学院名优茶现代化加工关键技术与设备科技创新团队(12C0803)

Research Advance on β-Glucosidase of Tea Plant

ZHOU Hanchen, LEI Pandeng, DING Yong*   

  1. Tea Research Institution, Anhui Academy of Agricultural Sciences, Huangshan 245000, China
  • Received:2015-08-13 Online:2016-04-15 Published:2019-08-23

摘要: 茶树体内的各种物质代谢、能量传递以及生长发育等都必须有酶的参与,而且酶对茶叶品质的形成具有重要意义。β-葡萄糖苷酶能够催化茶树糖苷类香气前体的水解反应,产生的茶树香气物质不仅参与茶树对病虫害的防御反应,还是茶叶重要的物质基础。茶树β-葡萄糖苷酶基因在表达部位、亚细胞定位、蛋白结构及系统发育进化等生物信息学方面,与拟南芥、水稻、玉米等植物的β-葡萄糖苷酶基因家族都存在相似性。在非生物和生物逆境胁迫下,茶树β-葡萄糖苷酶基因表达在逆境初期均呈现上调。本文将主要围绕茶树β-葡萄糖苷酶基因生物信息学及基因表达等研究现状,结合β-葡萄糖苷酶在茶叶加工中的变化作一概述。

关键词: 茶树, β-葡萄糖苷酶, 生物信息学, 基因表达

Abstract: All material metabolisms, energy transfer, growth and development in tea plant require the participation of enzymes, which are also of great importance for tea quality formation. β-glucosidase catalyzes the hydrolysis of tea glycoside precursors. The produced aroma substances are not only involved in plant defense reaction against diseases and pests, but also important for tea quality. The β-glucosidase of Camellia sinensis is highly similar to β-glucosidase gene family members of arabidopsis, rice and maize in expression sites, subcellular localization, protein structure and phylogenetic evolution. Under biotic and abiotic stresses, the β-glucosidase of Camellia sinensis is immediately induced in the early stage. This paper mainly focused on the bio-informatic and gene expression in tea plant studies of Camellia sinensis β-glucosidase. Meanwhile, the changes of β-glucosidase in tea processing were also reviewed.

Key words: tea plant(Camellia sinensis), β-glucosidase, bioinformatics, gene expression

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