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茶叶科学 ›› 2020, Vol. 40 ›› Issue (4): 536-543.doi: 10.13305/j.cnki.jts.20200612.003

• 研究报告 • 上一篇    下一篇

六堡茶茶褐素体外降脂功效研究

龚受基1, 滕翠琴2, 梁东姨1, 曹惠怡1, 蒙彦妃1, 张均伟3, 谢加仕3   

  1. 1.北部湾大学食品工程学院,广西 钦州 535011;
    2.梧州市农业科学研究所,广西 梧州 543000;
    3.梧州中茶茶业有限公司,广西 梧州 543000
  • 收稿日期:2019-10-08 修回日期:2019-12-02 出版日期:2020-08-15 发布日期:2020-08-18
  • 作者简介:龚受基,男,副教授,主要从事食品化学成分与功能关系方面的研究,gong5895801@163.com
  • 基金资助:
    广西高校科学技术研究项目(KY2015ZD086)、北部湾大学校级科研项目(2017KYQD223)

In Vitro Study on Hypolipidemic Effects of Theabrownins in Liupao Tea

GONG Shouji1, TENG Cuiqin2, LIANG Dongyi1, CAO Huiyi1, MENG Yanfei1, ZHANG Junwei3, XIE Jiashi3   

  1. 1. College of Food Engineering, Beibu Gulf University, Qinzhou 535011, China;
    2. Wuzhou Institute of Agricultural Sciences, Wuzhou 543000, China;
    3. China Tea (Wuzhou) Co., Ltd, Wuzhou 543000, China
  • Received:2019-10-08 Revised:2019-12-02 Online:2020-08-15 Published:2020-08-18

摘要: 考察六堡茶茶褐素体外结合胆酸盐能力、抑制胰脂肪酶活性及胆固醇酯酶活性能力、吸附胆固醇及油脂能力,从而评价其体外降脂活性。研究结果显示,六堡茶茶褐素对花生油的吸附量为0.73 g·g-1,对胆固醇的吸附量在酸性条件下为122.42 mg·g-1、中性条件下为13.68 mg·g-1;在茶褐素结合胆酸盐的试验中,随着茶褐素浓度的增加,与胆酸盐结合能力也呈上升趋势;茶褐素对胰脂肪酶起激活作用,且随着浓度的增加呈持续上升趋势;对胆固醇酯酶活性抑制IC50值为57.2 mg·mL-1。六堡茶茶褐素降脂机制可能主要通过与胆酸盐结合、吸附胆固醇和脂肪来实现。

关键词: 六堡茶, 茶褐素, 体外降脂, 胆酸盐

Abstract: To evaluate the hypolipidemic effects of theabrownins in Liupao tea, their abilities to bind cholate, inhibit pancreatic lipase and cholesterol esterase, adsorb cholesterol and lipid in vitro were determined. The results show that the adsorption capacity of peanut oil by theabrownins was 0.73 g·g-1, and the adsorption amount of cholesterol was 122.42 mg·g-1 under acid condition and 13.68 mg·g-1 under neutral condition. In the theabrownin-binding cholate test, the binding abilities to the cholate showed an upward trend with the increase of theabrownin concentrations. However, the figures indicate that theabrownins can activate pancreatic lipase and it continues to increase with the increase of concentration. The IC50 value of inhibition on cholesterol esterase activity was 57.2 mg·mL-1. The hypolipidemic mechanism of theabrownins in Liupao tea might be mainly achieved by binding to cholate and adsorbing cholesterol and lipid.

Key words: Liupao tea, theabrownin, hypolipidemic effects, cholate

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