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茶叶科学 ›› 2017, Vol. 37 ›› Issue (5): 513-522.

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外源接种黄曲霉污染普洱茶安全性研究

李亚莉, 邢倩倩, 涂青, 周红杰*   

  1. 云南农业大学普洱茶学院,云南 昆明 650201
  • 收稿日期:2017-03-18 修回日期:2017-05-23 出版日期:2017-10-25 发布日期:2019-08-23
  • 通讯作者: *1051195348@qq.com
  • 作者简介:李亚莉,女,博士,副教授,硕士生导师,主要从事普洱茶加工和综合利用研究。
  • 基金资助:
    国家自然科学基金项目(31460215)、云岭产业技术领军人才(发改委[2014]1782)

Study on the Safety of Pu-erh Tea Contaminated by Exogenous Aspergillus flavus

LI Yali, XING Qianqian, TU Qing, ZHOU Hongjie*   

  1. Pu-erh Tea College, Yunnan Agricultural University, Kunming 650201, China
  • Received:2017-03-18 Revised:2017-05-23 Online:2017-10-25 Published:2019-08-23

摘要: 以云南普洱茶为实验材料,外源接种黄曲霉(Aspergillus flavus)菌株于普洱茶原料及成品中,设未接种菌株普洱茶为对照,分别置于室温,湿度80%、温度30℃,湿度90%、温度30℃的恒温恒湿箱条件下存放,在第7天、14天、21天、28天分别取样以LC-MS/MS检测法进行黄曲霉毒素检测。检测结果表明,所有受试茶样中均未检测到黄曲霉毒素B1、B2、G1、G2,检出率为0。说明无论是室温还是在高温高湿条件下,被黄曲霉污染的普洱茶,经存放后,不会产黄曲霉毒素,就这一点而言普洱茶具有较高的饮用安全性。

关键词: 普洱茶, 黄曲霉, 黄曲霉毒素, 安全性

Abstract: Yunnan Pu-erh tea was used as experimental material. Aspergillus flavus strains YM31882 were inoculated into Pu-erh tea, and the Pu-erh tea un-inoculated by strains was taken as the control. The storage conditions were (1) temperature 30℃, humidity 90%. (2) temperature 30℃, humidity 90% and (3) room temperature. The tea samples were storage for 7, 14, 21and 28 days respectively and then collected for yellow aspergillus toxin detection by LC-MS / MS. The results showed that none of the aflatoxin B1, B2, G1, G2 was detected in tested samples. indicating that storage process could avoid Aspergillus flavus contamination in Pu-erh tea, In summary, Pu-erh tea is safe to drink in this field.

Key words: Pu-erh tea, Aspergillus flavus, aflatoxin, safety

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