茶树根际土壤真菌ALF-1（Neurospora sp.）耐酸铝基因片段克隆

doi:10.13305/j.cnki.jts.2004.01.008

Abstract

Abstract: Aluminum-tolerant associated gene of fungus ALF-1(Neurospora sp.) in rhizospheric soil of tea plant was cloned using rt-PCR technique. The gene expression was induced by aluminum treatment, and its sequence was 359 base pair (bp). The opening reading frame (ORF) was 260bp which situated from 100^th to 359^thbp, and encoded 86 amino acid residues. BLAST analysis showed that the gene sequence obtained was 50% identity to that of Arabidopsis thaliana (thale cress).

Key words: Aluminum-tolerant fungus, Aluminum-tolerant gene, Cloning

CLC Number:

F307.12

LU Jian-liang, LIANG Yue-rong, WU Ying, HUANG Fu-ping. Cloning of Aluminum-tolerant Associated Gene from Fungus ALF-1(Neurospora sp.) in Rhizospheric Soil of Tea Plant[J]. Journal of Tea Science, 2004, 24(1): 41-43.

References 9

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Cloning of Aluminum-tolerant Associated Gene from Fungus ALF-1(Neurospora sp.) in Rhizospheric Soil of Tea Plant

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