茶树鲜叶中叶黄素循环组分的高效液相色谱法测定研究及其光保护功能鉴定

doi:10.13305/j.cnki.jts.2004.01.012

Abstract

Abstract: The xanthophyll cycle components in fresh leaves of tea plant (Camellia sinensis cv. Fudingdabai)were extracted by acetone. The pigments were analyzed with a Spherisorb column （C₁₈ , 5μm,250㎜×4.6βmm）,and were eluted using acetonitrile: methanol(85:15) and methanol:ethyl acetate(68:32) by a linear gradient. The pigments were detected by their absorbance at 445βnm. It was showed that violaxanthin, antheraxanthin and zeaxanthin from fresh leaves of tea plant were detected by HPLC, respectively, under temperatures of 15℃, 22℃ and 30℃, meanwhile, the pigments were separated well, and it is a sensitive, well-reappeared method. Tea plant had a larger xanthophyll cycle pool and a larger de-epoxidation state in higher sun-light, which indicated xanthophyll cycle plays an important role in photo-protection in tea plant.

Key words: High performance liquid chromatography, Tea plant, Xanthophylls cycle component, Photo-protection

CLC Number:

S571.1

WEI Chao-ling, JIANG Chang-jun, TAO Han-zhi, HU Ying-hui. Analysis of the Xanthophyll Cycle Component in Fresh Leaves of Tea Plant by High Performance Liquid Chromatography and Its Photo-protection[J]. Journal of Tea Science, 2004, 24(1): 60-64.

References 8

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Analysis of the Xanthophyll Cycle Component in Fresh Leaves of Tea Plant by High Performance Liquid Chromatography and Its Photo-protection

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