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Journal of Tea Science ›› 2004, Vol. 24 ›› Issue (2): 129-134.doi: 10.13305/j.cnki.jts.2004.02.011

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Selection of Optimum Conditions for Theaflavin Transformation from Tea Polyphenol through Immobilized Enzyme Membrane

TU You-ying1, FANG Qing2, LIANG Hui-ling1, HUANG Hai-tao1   

  1. 1. Department of Tea Science, Zhejiang University, Hangzhou 310029, China;
    2. Shao Yi-fu Hospital, Zhejiang University, Hangzhou 310013, China
  • Received:2003-12-22 Revised:2004-03-19 Online:2004-06-25 Published:2019-09-16

Abstract: Immobilized polyphenol oxidases (IPPO) was applied for the synthesis of theaflavins using tea catechins as substrates. A Response Surface Methodological (RSM) study was conducted using a five-level, five-variable, central composite rotation design (CCRD) to select the optimum catalysis conditions for theaflavin transformation from tea polyphenol through immobilized enzyme membrane. Five important variables were considered, namely, incubation duration, enzyme/substrate ratio (E/S, Activity Units/ml-AU/ml), aeration volume, concentration of tea polyphenols, and the pH value of the reaction system. The highest theaflavins concentration of 0.766βmg/ml was predicted under the conditions as with 49βmin for the incubation duration, 128.7 for E/S ratio, 23.81βml/min ventilation for air volume, 5.95βmg/ml for catechins concentration, and pH 4.3. The measured theaflavins concentration was 0.754±0.017βmg/ml under the same condition, which suggested that the prediction model was practical and the selected conditions were reasonable.

Key words: Response surface method, Catechin, Theaflavins, Immobilized enzyme membrane, Polyphenol oxidases

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