Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (2): 133-140.doi: 10.13305/j.cnki.jts.2007.02.007

Previous Articles     Next Articles

Differential Gene Expression Profiles Analysis of Tea Plant Induced by Tea Looper (Ectropic oblique) Attack Using DDRT-PCR

WEI Chao-ling1, 2, *, GAO Xiang-feng1, YE Ai-hua1, YANG Yun-qiu1, JIANG Chang-jun1   

  1. 1. Key Laboratory of Tea Bio-chemistry and technology,Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China;
    2. School of Resources and Environmental Science, Anhui Agricultural University, Hefei 230036, China
  • Received:2007-02-08 Revised:2007-03-08 Online:2007-06-25 Published:2019-09-11

Abstract: DDRT-PCR technique was used to explore the differential gene expression profiles analysis of tea plant induced by tea looper (Ectropic oblique) attack. Results showed that 222 differential expression fragments were obtained, which was 32.8% of total fragments, by amplified in anchor primers A3, A4, A6, A7, A8 and random primers R1-R8,R12, respectively. Twenty fragments of which were re-amplified by RT-PCR. Based on the BLASTx search in GenBank, it were found that eight of the fragments shared no homology, five of the fragments shared homology with the unknown proteins, and seven of the fragments shared higher score homology with the known proteins, which can be divided into six groups related to photosynthetic pigment protein in photosystem II(C15), abiotic resistance induced protein(D22), glucoside metabolism enzyme(A45), nucleic acid and protein transcription and regulation factors(A12, D63), auxin-regulated protein(E94) and VIP2 protein(D73). The fragments of C15, A45, D22, D73 and E94 were firstly found in the studies of molecular mechanism of plant-insect interaction, up to day.

Key words: tea plant, Ectropic oblique, damage induction, differential display for gene expression profile

CLC Number: