Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (3): 243-247.doi: 10.13305/j.cnki.jts.2007.03.006

Previous Articles     Next Articles

Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain

MAO Qing-li1,2, SHI Zhao-peng2, LI Lin3, LIU Zhong-hua2, ZHU Qi2   

  1. 1. College of Life Science,Xiaogan University, Xiaogan 432100, China;
    2. Key Laboratory of Tea Science of National Ministry of Education, Changsha 410128, China;
    3. College of Life Science, South China Normal University, Guangzhou 510631, China
  • Online:2007-09-25 Published:2019-09-11

Abstract: This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.The results showed that the catechin (TC80), which MIC90 was 25~100 μg/ml, showed the obvious inhibition of Agrobacterium rhizogenes. This showed that the plenty of polyphenols in the tea plant was a key factor to restrict Agrobacterium rhizogenes-mediated transformation of tea plant. An anti-polyphenol strain R1000AP obtained by anti-polyphenol screening, which anti-polyphenol capability enhanced 10~40 times. The hairy root frequency induced by the strain increased by 46.5%(P<0.05)compared to the non-anti-polyphenol strain.

Key words: catechin, Agrobacterium rhizogenes, inhibitatory action, anti-polyphenol strain, screen, hairy root frequency

CLC Number: