Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (4): 302-306.doi: 10.13305/j.cnki.jts.2007.04.007

Previous Articles     Next Articles

(-)-epigallocatechin Gallate (EGCG) Enhance the Anticancer Activity Induced by Carmustine in vitro

HUANG Xiu-hua1, ZHANG Dan2, HAO Jin2, YANG Zu-jun1, REN Zheng-long1, *   

  1. 1. The Laboratory of Molecular Biology, College of Life Sicence and Technology, University of Electronic Science and Technology, Chengdu 610051, China;
    2. The Laboratory of Pharmacology, Sichuan Enwei Institute of Traditional Chinese Medicine, Enwei Group, Chengdu 610041, China
  • Received:2007-05-24 Revised:2007-08-27 Online:2007-12-25 Published:2019-09-11

Abstract: The effect of (-)-epigallocatechin gallate (EGCG) combined with carmustine on the growth inhibition and DNA damage of human lung carcinoma cell line A549 were evaluated by MTT assay and Comet assay repectively. The results showed that 50% inhibitory concentration (IC50) of carmustine to A549 human lung carcinoma cell line was decreased when combined with EGCG. The 50% inhibitory concentration (IC50) of carmustine alone to human lung carcinoma cell line A549 was 187.46βμg/ml, When the carmustine is combine with EGCG(25βμg/ml and 50βμg/ml, the concentration under which EGCG has no growth inhibition to A549), however, the IC50 value to A549 cell line decreased to 139.56βμg/ml and 154.02βμg/ml respectively. EGCG also enhanced carmustine-induced DNA damage in A549 cell line by Comet assay. The tail moment increased from 19.02±14.60 to 33.07±10.47. So, EGCG can enhance carmustine-induced DNA damage in A549 cell line, hence enhance the anticancer activity of carmustine.

Key words: EGCG, carmustine, DNA damage, comet assay, IC50

CLC Number: