EGCG与锌离子互作对PC-3细胞能量代谢的影响

doi:10.13305/j.cnki.jts.2009.4.001

Abstract

Abstract: In the present work, the effect of treatments with EGCG, Zn²⁺ and EGCG+Zn²⁺ on inducing apoptosis and content of adenylate nucleotide (ATP, ADP and AMP) in PC-3 cells was investigated by chromatin staining with Hoechst 33258 and HPLC. Hoechst 33258 staining revealed the appearance of condensed chromatin and fragmented apoptotic nuclei under fluorescence microscopy at a concentration of 80 µmol/L EGCG, 80 µmol/L Zn²⁺ and 80 µmol/L EGCG+80 µmol/L Zn²⁺ in PC-3 cells. In the determination of adenylate nucleotide, after treated with EGCG, Zn²⁺ and EGCG+Zn²⁺, content of total adenylate nucleotide, EC and ATP/ADP value were significantly decreased in PC-3 cells, which demonstrated that EGCG and Zn²⁺ have a markedly inhibitory effect on energy metabolism of PC-3 cells.

Key words: EGCG, Zn²⁺, PC-3 cells, apoptosis, adenosine triphosphate, energy metabolism

CLC Number:

SUN Shi-li, LING Cai-jin, YU Hai-ning, SHEN Sheng-rong, MIAO Ai-qing, PAN Shun-shun, PANG Shi, ZHUO Min, ZHAO Chao-yi. Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells[J]. Journal of Tea Science, 2009, 29(4): 251-256.

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Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells

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Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells