基于EST-SSR的福云（半）同胞系茶树品种（系）遗传多样性和亲缘关系分析

doi:10.13305/j.cnki.jts.2010.03.006

Abstract

Abstract: 40 half-sib tea cultivars related to Fuding Dabai and Yunnan Daye were used to analyze the genetic diversity and relationship based on EST-SSR markers. Totally 99 alleles were amplified using the 28 pairs of EST-SSR primers, the number of alleles per primer ranged from 2 to 6, on average of 3.54. The polymorphism information content (PIC) varied from 0.13 to 0.79, on average of 0.59. The expected heterozygosity (He) varied from 0.08 to 0.84 and the observed heterozygosity (Ho) ranged from 0.08 to 1.00, on average of 0.59 and 0.71, respectively. The Shannon index of the population was 1.14. The 40 half-sib tea cultivars were classified into 4 groups with the similarity coefficient at 0.55. The results showed that the genetic polymorphism of half-sib tea cultivars related to Fuding Dabai was more abundant than those of Yunnan Daye.

Key words: tea, half-sib, EST-SSR, genetic polymorphism, genetic relationship

CLC Number:

S571
Q32

YU Ji-zhong, HUANG Hai-tao, YAO Ming-zhe, YANG Ya-jun. Analysis of Genetic Diversity and Relationship of Half-sib Tea Cultivars Related to Fuding Dabai and Yunnan Daye Using EST-SSR Markers[J]. Journal of Tea Science, 2010, 30(3): 184-190.

References

[1] Gupta P K, Rustgi S, Sharma S, et al. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat[J]. Molecular Genetics and Genomics, 2003(270): 315-323.
[2] Yanik B, Jun Z, Dainis R, et al. Characterization of EST-SSRs in loblolly pine and spruce, Tree Genetics and Genomes[J]. 2007(3): 251-259.
[3] 王家麟, 孙佳莹, 于清岩, 等. EST-SSRs的开发及应用研究进展[J]. 生物信息学, 2006, 5(3): 140-142.
[4] Eujayl I, Sorrells M E, Baum M, et al. Assessment of genotypic variation among cultivated durum wheat based on EST-SSRs and genomic SSRs[J]. Euphytica, 2001(119): 39-43.
[5] Gao L F, Tang J F, Li H W, et al. Analysis of microsatellites in major crops assessed by computational and experimental approaches[J]. Molecular Breeding, 2003(12): 245-261.
[6] 金基强, 崔海瑞, 龚晓春, 等. 用EST-SSR标记对茶树种质资源的研究[J]. 遗传, 2007, 29(1): 103-108.
[7] 刘振, 王新超, 赵丽萍, 等. 基于EST-SSR 的西南地区茶树资源遗传多样性和亲缘关系分析[J]. 分子植物育种, 2008, 6(1): 100-110.
[8] 叶乃兴. 我国茶树育种的骨干亲本及其系谱分析[J]. 中国茶叶, 2008, 29(4): 11-13.
[9] 陈亮, 陈大明, 高其康, 等. 茶树基因组DNA提取与鉴定[J]. 茶叶科学, 1997, 17(2): 177-181.
[10] Ghislain M, Andrade D, Rodríguez F, et al. Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja group using RAPDs and nuclear SSRs[J]. Theoretical and Applied Genetics, 2006(113): 1515-1527.
[11] Botstein D, White R L, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 1980(32): 314-331.
[12] Martinez L E, Cavagnaro P F, Masuelli R W, et al. SSR-based assessment of genetic diversity in south American Vitis vinifera varieties[J], Plant Science, 2006, 170: 1036-1044.
[13] Prevost A, Wilkinson M J.A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars[J]. Theor Appl Genet, 1999(98): 107-112.

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Analysis of Genetic Diversity and Relationship of Half-sib Tea Cultivars Related to Fuding Dabai and Yunnan Daye Using EST-SSR Markers

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