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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (5): 350-354.doi: 10.13305/j.cnki.jts.2010.05.004

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Isolation and Identification of “Jinhua” Fungi from the Loose Tea with“Fungus Growing”

HUANG Hao, LIU Zhong-hua*, HUANG Jian-an, LI Shi, LI Juan, WU Yang, DENG Ting-ting, GONG-Xue   

  1. National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanicals, Key Lab of Tea Science of Ministry of Education of Hunan Agricultural University, Changsha 410128, China
  • Received:2010-04-22 Revised:2010-06-17 Published:2019-09-11

Abstract: A fungi called “Jinhua” that separated from Jing Xiangyi brick tea was produced by Hunan Yiyang Tea Factory in 2008. Inoculating the fungi to the raw material of Yiping Fuzhuan tea of 2008 with “Fungus growing” by controlling the conditions of environment. A strain of “Jinhua” fungi was isolated from the loose tea with fungus growing as a tested fungi through subculture purification. The epigamous and phorozoon of the tested fungi was observed with microscope, and the ascospores, and conidia of the tested fungi was observed SEM, and the tested fungi was identified in the molecular level by DNA sequencing. According to the characteristic of the morphology, microscopic, and SEM observed as well as the DNA sequencing of the tested strains, it was identified as Eurotium cristatum.

Key words: fungus growing, “Jinhua” fungi;, microscope, scanning electron microscope, Eurotium cristatum

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