低磷胁迫下茶树根系CS基因的克隆及表达分析

doi:10.13305/j.cnki.jts.2010.05.006

Abstract

Abstract: Citrate synthase(CS) is the key enzyme of producing citrate acid. CS gene fragments were cloned from tea roots. The GenBank accession is FJ814766 in the NCBI. Then the changes in gene expression in response to P supply were investigated by using semi-quantitative RT-PCR. The result showed that P deficiency increased the expressions of CS gene. This may be a tolerant mechanism of tea seedlings to P deficiency.

Key words: semi-quantitative RT-PCR, low P stress, gene expression, cloning, tea plant

CLC Number:

S571
Q523

LIN Zheng-he, CHEN Chang-song, WU Ling-sheng. Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress[J]. Journal of Tea Science, 2010, 30(5): 362-366.

References

[1] Kochian LV, Hoekenga OA, Pineros MA.How do crop plants tolerate acid soils? Mechanism of aluminum tolerance and phosphorus efficiency[J]. Annu Rev Plant Biol, 2004,55: 459-493.
[2] Yan X, Wu P, Ling H, et al. Plant nutriomics in China: an overview[J]. Ann Bot, 2006, 98(3): 473-482.
[3] Epstein E, Better, EPSTEIN E.Crop tolerance to salinity and other mineral stresses[C]//Nugent J, O'Conner M. Better Crops for Food. London: Pitman, 1983: 61-68.
[4] Vance CP, Uhde-Stone C, Allan DL.Phosphorus acquisition and use critical adaptations by plants for securing a nonrenewable resource[J]. New Phytol, 2003, 157(3): 423-447.
[5] 李庆逵, 蒋柏藩, 鲁如坤. 中国磷矿的农业应用[M]. 江苏: 江苏科技出版社, 1992.
[6] Jemo M, Abaidoo RC, Nolte G, et al. Aluminum resistance of cowpea as affected by phosphorus-deficiency stress[J]. J Plant Physiol, 2007, 164(4/5): 442-451.
[7] Johnson JF, Vance CP, Allan DL.Phosphorus deficiency in Lupinus albus: Altered Lateral root development and enhanced expression of phosphoenolpyruvate carboxylase[J]. Plant Physiol, 1996, 112(1): 31-41.
[8] Neumann C, Massonneau A, Martinoia E, et al. Physiological adaptations to phosphorus deficiency during proteoid root development in white lupin[J]. Planta, 1999, 2008(3): 373-382.
[9] Ligaba A, Yamaguchi M, Shen H, et al. Phosphorus deficiency enhances plasma membrane H+-ATPase activity and citrate exudation in greater purple lupin (Lupinus pilosus)[J]. Funct Plant Biol, 2004, 31(11): 1075-1083.
[10] Hoffland E, Findenegg GR, Nelemans JA.Solubilization of rock phosphate by rape. II. Local root exudation of organic acids as a response to P-starvation[J]. Plant Soil, 1989, 113(2): 161-165.
[11] Faezeh G, Akio M, Hiromi Y.Effects of aluminum on the growth of tea plant and activation of antioxidant system[J]. Plant Soil, 2005, 276(1/2): 133-141.
[12] Salehi SY, Hajiboland R.A high internal phosphorus use efficiency in tea (Camellia sinensis L.) plants[J]. Asian J Plant Sci, 2008, 7(1): 30-36.
[13] Wan CY, Wilkins TA.A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.)[J]. Anal Biochem, 1994, 223(1): 7-12.
[14] Hanning I, Heldt H W.On the function of mitochondrial metabolism during photosynthesis in spinach leaves(Spinacia olerocea L) leaves (Partitioning between respiration and export of redox equivalents and precusors for nitrate assimilation products)[J]. Plant Physiology, 1993, 103(4): l147-1l54.
[15] Johnson J F, Allan D L, Vance C P, et al. Root carbon dioxide fixation by phosphorus-deficient Lupinus albus: Contribution to acid exudation by organic-proteid roots[J]. Plant Physiology, 1996, 112(1): 19-30.
[16] Johnson J F, Allan D L, Vance C P.Phosphorus stress-induced proteoid roots show altered Metabolism in Lupinus albus[J]. Plant physiol, 1994, 104(2): 657-665.
[17] Koyama H, Takita E, Kawamura A, et al. Over Expression of Mi-tochondrial Citrate Synthase Gene improves the Growth of Carrot Cells in Al-Phosphate Medium[J]. Plant Cell Physiol, 1999, 40(5): 482-488.
[18] De la Fuente JM, Ramirez-Rodriguez V, Cabrerra-Ponce JL, et al. Aluninum tolerance in transgenic plants by alteration of citrate synthesis[J]. Science, 1997, 276(5318): 1566-1568.
[19] López-Bucio J, Nieto-Jacobo MF, Ramírez-Rodríguez V, et al. Organic acid metabolism in plants: from adaptive physiology to transgenic varieties for cultivation in extreme soils[J]. Plant Sci, 2000, 160(1): 1-13.
[20] 李亚青, 葛荣朝, 赵保存, 等. RT-PCR法半定量检测不同逆境胁迫下TaGSK1基因的表达[J]. 华北农学报, 2007, 22(1): 83-85.
[21] 汪以真, 林文学, 许梓荣. 半定量RT-PCR法评定不同生长阶段猪抗菌肽pr-39基因表达差异[J]. 农业生物技术学报, 2004, 12(1): 52-55.

Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[2]	SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors [J]. Journal of Tea Science, 2022, 42(5): 689-704.
[3]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[4]	LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants [J]. Journal of Tea Science, 2022, 42(3): 347-357.
[5]	YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress [J]. Journal of Tea Science, 2022, 42(2): 187-199.
[6]	CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea [J]. Journal of Tea Science, 2022, 42(1): 18-28.
[7]	TANG Lei, XIAO Luodan, HUANG Yifan, XIAO Bin, GONG Chunmei. Cloning and Magnesium Transport Function Analysis of CsMGTs Genes in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(6): 761-776.
[8]	WU Wanfu, LYU Shidong, YANG Xuefang, LI Yue, ZHANG Jiguang. Study of Characteristic Indexes and Fatty Acid Composition in Big-leaf Tea Plant (Camellia sinensis var. assamica) Seed Oils from Yunnan Province [J]. Journal of Tea Science, 2021, 41(4): 463-470.
[9]	LIU Dingding, WANG Junya, TANG Rongjin, CHEN Liang, MA Chunlei. Genome-wide Identification of PPR Gene Family and Expression Analysis of Albino Related Genes in Tea Plants [J]. Journal of Tea Science, 2021, 41(2): 159-172.
[10]	ZHANG Qunfeng, NI Kang, YI Xiaoyun, LIU Meiya, RUAN Jianyun. Advances of Magnesium Nutrition in Tea Plant [J]. Journal of Tea Science, 2021, 41(1): 19-27.
[11]	CHEN Yao, ZHANG Weifu, REN Hengze, XIONG Fei, ZHANG Haojie, YAO Lina, LIU ying, WANG Lu, WANG Xinchao, YANG Yajun, HAO Xinyuan. Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant (Camellia Sinensis) [J]. Journal of Tea Science, 2021, 41(1): 28-39.
[12]	JIN Ke, HUANG Jian'an, XIONG Ligui, LIU Shuoqian, QIN Xiaohong, PENG Jing, LI Yinhua, LI Juan. Research of Theanine-related Genes Expressed in Etiolated Tea Plant (Camellia Sinensis) [J]. Journal of Tea Science, 2021, 41(1): 40-47.
[13]	WAN Qiwen, YANG Ni, LI Yimin, HAN Miaohua, LIN Shijia, TENG Ruimin, ZHUANG Jing. Effects of Exogenous 24-Epibrassinolide on Photosynthetic Characteristics of Tea Plants [J]. Journal of Tea Science, 2021, 41(1): 58-70.
[14]	HUANG Fangfang, LI Qin, HUANG Jian'an. Research Progress of Tea Rhizosphere Microorganisms [J]. Journal of Tea Science, 2020, 40(6): 715-723.
[15]	FENG Xia, DI Taimei, PENG Jing, LI Nana, YAO Lina, YANG Yajun, WANG Xinchao, WANG Lu. Interaction Identification and Expression Analysis of CsCIPK12 and CsKIN10 in Tea Plant [J]. Journal of Tea Science, 2020, 40(6): 739-750.