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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (4): 295-299.doi: 10.13305/j.cnki.jts.2011.04.010

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Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips

WANG Jun-ping, ZHENG Chang-ying*   

  1. College of Agronomy and Plant Protection, Qingdao Agricultural university, Qingdao 266109, China
  • Received:2011-03-03 Revised:2011-03-28 Online:2011-08-15 Published:2019-09-09

Abstract: 12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality. CYT10 strain was identified based on the morphological and molecular characteristics. Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced. The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana (>95%). Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain, which was belonging to B. bassiana. CYT10 strain was classified as a member of the species B. bassiana based on the morphological characteristics and analysis of DNA sequences.

Key words: Beauveria bassiana, thrip, morphological characteristics, rDNA-ITS, EF1-a

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