遮荫对茶树新梢叶绿素及其生物合成前体的影响

doi:10.13305/j.cnki.jts.2012.02.011

Abstract

Abstract: Effects of light-shading on accumulation of chlorophylls and their biosynthetic precursors in tea cultivars Jiukeng, Longjing43, Shuigu were investigated using dark light-shading net. It showed that light-shading increased the accumulation of chlorophylls in tea shoots, accompanying decrease in levels of aminolevulinic acid (ALA), Porphobilinogen (PBG) and urogen Ⅲ and increase in levels of protoporphyrin Ⅸ (Proto Ⅸ), magnesium protoporphyrin Ⅸ (Mg-Proto Ⅸ) and protochlorophyllide (Pchlide).

Key words: tea shoot, chlorophylls, biosynthesis, precursors, shading, low illumination intensity

CLC Number:

SHU Hua, WANG Ying-feng, ZHANG Shi-kang, LIANG Yue-rong, LU Jian-liang, LI Da-wei, OU Yang-mei, LI Na-na, ZHU Yue-jin. Effect of Light-shading on Accumulation of Chlorophylls and Their Precursors in Tea Shoots[J]. Journal of Tea Science, 2012, 32(2): 115-121.

References

[1] 何锦凤, 郝利民. 论膳食纤维[J]. 食品与发酵工业, 1997, 23(5): 63-68.
[2] 刘本英, 王平盛. 茶多酚生物学活性的研究进展[J]. 中国农学通报, 2005, 21(12): 92-94.
[3] 吕毅, 郭雯飞, 倪捷儿. 茶氨酸的生理作用及合成[J]. 茶叶科学, 2003, 23(1): 1-5.
[4] 潘顺顺, 陆建良, 杨晓丽, 等. 茶饮料中微量色素物质的HPLC分析方法研究[J]. 茶叶科学, 2007, 27(4): 343-348.
[5] Roger L, Hideo T.Effect of Benzyladenine Treatment Duration on B-AminolevuliniicAcid Accumulation in the Dark, Chlorophyll Lag Phase Abolition,and Long-Term Chlorophyll Production in Excised Cotyledons of Dark-Grown Cucumber Seedlings[J]. Plant Physiol, 1982, 69: 663-667.
[6] 孙捷音, 张年辉, 杜林方. 油菜叶绿素b减少突变体Cr3529叶绿素生物合成的研究[J]. 西北植物学报, 2007, 27(10): 1962-1966.
[7] Rebeiz CA, Mattheis JR, Smith BB, et al. Chloroplast biogenesis. Biosynthesis and accumulation of protochlorophyll by isolated etioplasts and developing chloroplasts[J]. Arch Biochem Biophys, 1975, 71(2): 549-567.
[8] Bogorad L.Porphyrin synthesis[A]. Methods in enzymology[C]. NewYork Academic Press, 1962: 885-895.
[9] Lee H J, Ma rk DB, Parham R, et al. Chloroplast biogenesis 65: Enzymic Conversion of Protoporphyrin IX to Mg-Protoporphyrin IX in a Subplastidic Membrane Fraction of Cucumber Etiochloroplasts[J]. Plant Physiology, 1992, 99: 1134-1140.
[10] 袁志发, 周静芋. 试验设计与分析[M]. 北京: 高等教育出版社, 2001: 210-232.
[11] 肖润林, 王久荣, 单武雄, 等. 不同遮荫水平对茶树光合环境及茶叶品质的影响[J]. 中国生态农业学报, 2007, 15(6): 7-11.
[12] 张文锦, 梁月荣, 张方舟, 等. 覆盖遮荫对乌龙茶产量品质的影响[J]. 茶叶科学, 2004, 24(4): 276-282.
[13] 白伟岚, 任建武, 苏雪痕. 八种植物耐荫性比较研究[J]. 北京林业大学学报, 1999, 21(3): 46-51.
[14] 岳桦, 孙颖. 三种室内观花植物的耐荫性研究[J]. 北方园艺, 2004(2): 39.
[15] 白伟岚. 园林植物的耐荫性研究[J]. 林业科技通讯, 1999(2): 12-15.
[16] Boardman N K.Compearative photosynthesis of sun and shade plants[J]. Annu Rev Plant Physiology, 1997, 28: 355-371.
[17] Armstrong G A, Runge S, Frick G, et al. Identification of NADPH: protochlorophyllide oxidoreductases A and B:a branched pathway for light-dependent chlorophyll biosynthesis inArabidopsis thaliana[J]. Plant Physiol, 1995, 108(4): 1505-1517.
[18] Oosawa N, Masuda T, Awai K, et al. Identification and light-induced expression of a novel gene of NADPH- protochlorophyllide oxidoreductase isoform inArabidopsis thaliana[J]. FEBS Lett, 2000, 474(2/3): 133-136.
[19] Benli M, Schulz R, Apel K.Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana[J]. Plant Mol Biol, 1991, 16(4): 615-625.
[20] Ilag LL, Kumar AM, Soll D.Light regulation of chlorophyll biosynthesis at the level of 5-aminolevulinate formation in Arabidopsis[J]. Plant Cell, 1994, 6(2): 265-275.
[21] 王平荣, 张帆涛, 高家旭, 等. 高等植物叶绿素生物合成的研究进展[J]. 西北植物学报, 2009, 29(3): 629-636.
[22] Kumar AM, Csankovszki G, Soll D.A second and differentially expressed glutamyl-tRNA reductase gene from Arabidopsis thaliana[J]. Plant Mol Biol, 1996, 30(3): 419-426.
[23] Lim SH, Witty M, Wallacecook AD, et al. Porphobilinogen deaminase is encoded by a single gene in Arabidopsis thalianaand is targeted to the chloroplasts[J]. Plant Mol Biol, 1994, 26(3): 863-872.
[24] Narita S, Tanaka R, Ito T, et al. Molecular cloning and characterization of a cDNA that encodes protoporphyrinogen oxidase of Arabidopsis thaliana[J]. Gene, 1996, 182: 169-175.
[25] Sangwan I, O’brain M R.Expression of a soybean gene encoding the tetrapyrrole-synthesis enzyme glutamyl-tRNA reductase in symbiotic root nodules[J]. Plant Physiol, 1999, 119(2): 593-598.
[26] Kaczor CM, Smith MW, Sangwan I, et al. Plant delta-aminolevulinic acid dehydratase (Expression in soybean root nodules and evidence for a bacterial lineage of the Alad gene)[J]. Plant Physiol, 1994, 104(4): 1411-1417.
[27] Madsen O, Sandal L, Sandal NN, et al. A soybean coproporphyrinogen oxidase gene is highly expressed in root nodules[J]. Plant Mol Biol, 1993, 23(1): 35-43.
[28] Witty M, Wallace-cook AD, Albrecht H, et al. Structure and expression of chloroplast-localized porphobilinogen deaminase from pea (Pisum sativumL.) isolated by redundant polymerase chain reaction[J]. Plant Physiol, 1993, 103(1): 139-147.
[29] Mock HP, Trainotti L, Kruse E, et al. Isolation sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley[J]. Plant Mol Biol, 1995, 28(2): 245-256.
[30] Kruse E, Mock HP, Grimm B.CoproporphyrinogenⅢoxidase from barley and tobacco--sequence analysis and initial expression studies[J]. Planta, 1995, 196(4): 796-803.
[31] Lermontova I, Kruse E, Mock HP, et al. Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase[J]. Proc Natl Acad Sci USA, 1997, 94(16): 8895-8900.

Effect of Light-shading on Accumulation of Chlorophylls and Their Precursors in Tea Shoots

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[2]	JIAO Haizhen, SHAO Chenyu, CHEN Jianjiao, ZHANG Chenyu, CHEN Jiahao, LI Yunfei, SHEN Chengwen. Dynamic Changes of Physiological Responses and Antioxidant Enzyme Activities in Tea Root System under Severe Shading and Removal of Shading [J]. Journal of Tea Science, 2021, 41(5): 695-704.
[3]	XU Lijia, LIU Qi, DAI Jianwu, CHEN Xiaohu. Design of End Effector for Picking Tea Shoots [J]. Journal of Tea Science, 2021, 41(5): 705-716.
[4]	WANG Guofu, SUN Xiaohong, FANG Yi, ZHOU Jin, SHEN Qianting, XIANG Junlei, JIN Xianling, LUO Xingyun. Effects of Shading on Microbial Characteristics and Enzyme Activities in Matcha Tea Garden Soil [J]. Journal of Tea Science, 2019, 39(3): 355-363.
[5]	PANG Dandan, ZHANG Fen, ZHANG Yazhen, WEI Kang, WANG Liyuan, CHENG Hao. Research Advance on Biosynthesis, Regulation and Function of Anthocyanins in Tea Plant [J]. Journal of Tea Science, 2018, 38(6): 606-614.
[6]	XIE Yunhai, ZHENG Deyong, YE Naixing, JIN Shan, ZENG Puyu. Analysis on the Contents of Zhangping Shuixian Tea’s Aroma Precursors during Manufacturing Processes [J]. Journal of Tea Science, 2016, 36(1): 11-17.
[7]	WEI Kang, WANG Liyuan, CHENG Hao, ZHANG Liqun, FENG Suhua. Analysis of Differential Gene Expression on Flavonoid Biosynthesis and Its Regulation in Tea Plant (Camellia sinensis L.) [J]. Journal of Tea Science, 2014, 34(2): 195-203.
[8]	WEI Jia-jia, CHEN Yong, JIN Xiao-jun, ZHENG Jia-qiang, SHI Yuan-zhi, ZHANG Hao. Researches on Tender Tea Shoots Identification under Natural Conditions [J]. Journal of Tea Science, 2012, 32(5): 377-381.
[9]	CHEN Lin, ZHANG Zheng-zhu, CHEN Jian, ZHANG Ying-gen, WAN Xiao-chun. Review on Enzymatic Biosynthesis of Theanine [J]. Journal of Tea Science, 2011, 31(1): 1-10.
[10]	WANG Bin, JIANG He-yuan, ZHANG Jiang-yong, WANG Yan, HUANG Yong-dong. Application of Enzyme Engineering Technology in Tea Deep-processing and Its Perspectives [J]. Journal of Tea Science, 2010, 30(S1): 521-526.
[11]	ZHANG Xian-lin, GAO Li-ping, XIA Tao, LIU Ya-jun, GAO Ke-jun. Study on the Changes of Non-galloylated Catechins and Relative Enzymes in Tea Shoots [J]. Journal of Tea Science, 2009, 29(5): 365-371.
[12]	WANG Li-yuan, WANG Xian-bo, CHENG Hao, ZHOU Jian. Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli [J]. Journal of Tea Science, 2007, 27(2): 111-116.
[13]	YU Ji-hong, HUA Dong, HUA Ping, LENG He-ping, JIANG Shao-mei, LU Hu. Study on Optimum Medium for Theanine Biosynthesis in Suspension Cultures of Camellia sinensis [J]. Journal of Tea Science, 2006, 26(2): 131-135.
[14]	ZHANG Zheng-Zhu, WAN Xiao-Chun, TAO Guang-Jun. Analysis of Glycosidic Aroma Precursors in Fresh Tea Leaves (Camellia sinensis var. sinensis cv. Zhuye) by the Combination of Liquid Chromatography with Mass Spectrometry [J]. Journal of Tea Science, 2005, 25(4): 275-281.
[15]	Edward G Mamati, LIANG Yue-rong. Genetic Control Studies to Exploit Flavonoids Synthesis Pathway in Tea Biochemical Improvement [J]. Journal of Tea Science, 2005, 25(2): 81-89.