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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (6): 539-542.doi: 10.13305/j.cnki.jts.2012.06.004

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Protective Effect of Fuzhuan Brick Tea on H2O2-Induced Cellular Oxidative Damage in LLC-PK1 Cells

SONG Jia-le   

  1. Department of Food Science and Nutrition, Pusan National University, Pusan 609-735, South Korea
  • Received:2012-06-04 Revised:2012-08-06 Online:2012-12-15 Published:2019-09-05

Abstract: The aim of this study is to investigate the protective effect of ethanolic extract from Fuzhuan brick tea (FTE) on H2O2-induced oxidative damage in LLC-PK1 cells. Various concentration (10~200μg/mL) of FTE were pre-incubated with LLC-PK1 cells for 24h, and then exposed to H2O2 (500μmol/L) for 4h. The cell viability was determined by MTT assay, contents of malondialdehyde (MDA) were determined by thiobarbituric acid reactive substances (TBARS) assay. Intracellular antioxidant enzyme activities, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were determined by colorimetric assay according to the protocols of the commercial kit. The cell viabilities of H2O2 treated LLC-PK1 cells were increased, and the contents of MDA were decreased after 24h pre-incubated with different concentrations of FTE. In addition, the antioxidant enzymes (CAT, SOD and GSH-px) levels were also increased in FTE compared with H2O2 treated group. There was statistical significant difference between the FTE treated groups and untreated H2O2 treated groups (all P<0.05). Those results suggested that pre-incubated with FTE showed a protective effect against H2O2-induced oxidative damage in LLC-PK1 cells.

Key words: Fuzhuan brick tea, oxidative damage, antioxidant, LLC-PK1 cells

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