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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (3): 230-238.doi: 10.13305/j.cnki.jts.2014.03.004

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Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes

TENG Cuiqin1,2, LIU Zhonghua1,2,*, GONG Shouji3, PENG Yuxuan4, Ma Rui5   

  1. 1. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    2. Key Lab of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
    3. College of Pharmacy, Guilin Medical University, Guilin 541004, China;
    4. Dark Tea Studies Insitute, Hunan City University, Yiyang 413000, China;
    5. Guangxi Vocational & Technical College, Nanning 530226, China
  • Received:2013-07-12 Revised:2013-09-16 Online:2014-06-15 Published:2019-09-03

Abstract: To investigate the effect of Liupao tea on glucose and lipid metabolism in palmitate-induced insulin resistance 3T3-L1 adipocytes. Glucose intake decreased and insulin resistance was induced in 3T3-L1 adipocytes after incubation in 1mmol/L palmitate for 24 hours. Compared with the model group, the concentrations of glucose and NEFA decreased along with the increase of Liupao tea concentrations in medium. The mRNA expression of key enzyme for glycolysis of glucose (Hexokinase, 6-phosphofructokinase-1, and pyruvatekinase) and the Lipid-Metabolism-Related Enzymes (Sn-glycerol-3-phosphate acyltransferase) significantly increased in the Liupao tea groups comparing with model sample. Meanwhile the glucose transporter 4 mRNA expression was also increased and the acetyl-CoA carboxylase, protein tyrosine phosphatase 1B mRNA expression was decreased. Low doses of Liupao tea reduced carnitine palmitoyl transferase I mRNA expression while high doses significantly raised it. Research proved that Liupao tea were able to increase glucose uptake and activate the glucolipid metabolic pathways. Liupao tea showed the effect of improving insulin resistance induced by palmitate in 3T3 -L1 adipocytes.

Key words: Liupao tea, glucolipid metabolism, insulin resistance, 3T3-L1 adipocytes

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