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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (6): 577-582.doi: 10.13305/j.cnki.jts.2014.06.019

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Cloning and Sequence Analysis of CsAP2 Gene from Tea Plant[Camellia sinensis (L.) O. Kuntz]

FANG Chenggang, XIA Lifei, CHEN Linbo*, TIAN Yiping, BAO Yunxiu, SHANG Weiqiong   

  1. Tea Research Institute, Yunnan Academy of Agricultural Science, Yunnan Engineering Research Center of Tea Germplasm Innovation and Matching Cultivation, Menghai 666201, China
  • Received:2014-04-25 Revised:2014-06-13 Online:2014-12-20 Published:2019-09-03

Abstract: The cDNA-AFLP was applied to identify genes expressed differentially between young and mature leaves of tea plant (Camellia sinensis var. assamica, cultivar Zijuan). A cDNA fragment, TDF encoding a APETALA2 was isolated, and containing a complete coding sequence cDNA was cloned by RACE, which encodes a polypeptide of 518 amino acids including two conserved AP2 domains, named CsAP2, sequence alignment showed that CsAP2 protein shared high identity with other plants. APETALA2 gene cloning provided foundation for studying flower development in tea plant.

Key words: Camellia sinensis, APETALA2 transcription factor, gene clone, sequence analysis

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