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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5): 469-476.doi: 10.13305/j.cnki.jts.2016.05.004

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Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC

LIU Zunying1,2, LIU Qiuling1,2, DENG Yanli1,2, CHEN Ling1,2, XIAO Wenjun1,2,3,*   

  1. 1. Key Lab of Tea Science of Ministry of Education, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China
  • Received:2016-03-24 Online:2016-10-15 Published:2019-08-26

Abstract: SPF grade Balb/c female mice were used as experimental animals. After adaptive feeding for 3 days, mice were orally pre-treated with different dosages of L-theanine once daily for 30 days before ETEC E44813 intraperitoneal injection. The body weight change in mice was analyzed during the breeding process. Mice were scarified 5 hours after ETEC injection and tissues were collected for analysis. The activities of ileum Glutathione peroxidase (GSH-Px), homogenate Catalase (CAT), Malondialdehyde (MDA) and Inducible nitric oxide synthase (iNOS) were measured and the pathological changes of intestinal and the levels of serum Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β) and Intercellular cell adhesion molecule-1(ICAM-1) were examined. This study was

Key words: L-theanine, Enterotoxigenic Escherichia coli., immune stress, intestinal protection

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