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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5): 537-543.doi: 10.13305/j.cnki.jts.2016.05.013

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A Primary Study on Sex Pheromone of Ectropis grisescens

LUO Zongxiu1,2, LI Zhaoqun1, CAI Xiaoming1, BIAN Lei1, CHEN Zongmao1,*   

  1. 1. Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-03-24 Online:2016-10-15 Published:2019-08-26

Abstract: Ectropis grisescens Warren is a severe defoliator species in tea garden of China. In this study, the sex pheromone was extracted from abdominal tips of virgin female moths by n-hexane during peak calling period. Analysis of gland extracts by gas chromatography (GC) with electroantennographic detection (EAD) and the components of sex pheromone were identified by gas chromatography (GC) coupled to mass spectrometry (MS). Finally, the biological activity of synthetic sex pheromone was evaluated by field trapping. The result of GC-EAD revealed two components that elicited responses from male moth antennae. Based on the mass spectrum, two components were identified as (Z,Z,Z)-3,6,9-octadecatriene and (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene. In field experiment, different attracting effects were identified under different ratio of sex pheromones. Experiment showed the ratio of 4:6 of these synthetic components showed the maximum attractiveness at 1.0 mg when loaded on rubber septum. This investigation provides a theoretical basis for developing sex pheromone application technology againest Ectropis grisescens.

Key words: Ectropis grisescens, sex pheromone, identification

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