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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (3): 347-357.doi: 10.13305/j.cnki.jts.2022.03.001

• Research Paper • Previous Articles     Next Articles

Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants

LIU Renjian1, WANG Yuyuan1, LIU Shaoqun1, SHU Canwei2, SUN Binmei1, ZHENG Peng1,*   

  1. 1. College of Horticulture, South China Agricultural University, Guangzhou 510642, China;
    2. College of Agriculture, South China Agricultural University/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Integrative Microbiology Research Center, Guangzhou 510642, China
  • Received:2021-12-18 Revised:2022-01-03 Online:2022-06-15 Published:2022-06-17

Abstract: Tea plant leaf trichomes contain various secondary metabolites and play an important role in the tea appearance quality as well as the response of tea plants to biotic and abiotic stresses. In this study, the function of leaf trichome-related genes CsbHLH024 and CsbHLH133 were analyzed using Bimolecular Fluorescent Complimentary (BiFC), GUS staining and overexpression experiments. The results show that CsbHLH024/CsbHLH133 and CsTTG1 could interact in plants, and their promoters could drive downstream gene expression in leaf tissues. They were further transformed into wild Arabidopsis thaliana (Col) and corresponding homozygous mutants, respectively to get overexpression lines. Both genes could affect the leaf trichome formation in Arabidopsis thaliana, restore the phenotype of the mutants, and induce the expression levels of trichome-related genes. This study provided a theoretical basis for further research on the molecular regulation mechanism of trichome formation in tea leaves.

Key words: tea plant, trichome formation, CsbHLH024, CsbHLH133, molecular regulation

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