茶树牻牛儿基牻牛儿基焦磷酸合成酶基因CsGGDPS的克隆及表达分析

Abstract

Abstract: A full-length cDNA sequence encoding geranylgeranyl diphosphate synthase (GGDPS) was isolated from transcriptome database of tea plant, cloned from C. sinensis cv. Tieguanyin and named as CsGGDPS. The cDNA length of CsGGDPS was 1β661βbp, with an open reading frame (ORF) of 1β137βbp and deduced protein of 378 amino acids. The protein was deduced to contain 5 conserved domains with 2 functional domains of Isoprenoid-Biosyn-C1 superfamily. The sequence analysis showed that CsGGDPS was highly conserved and had the closest genetic relationship with Panax notoginseng. CsGGDPS was an instability and hydrophilic protein, which was predicted to be located in chloroplast but with no transmembrane structure and signal peptide. There were 20 phosphorylation sites within the polypeptide chain. Alpha helix was predicted to be the major secondary structure of CsGGDPS. The three-dimension structure of CsGGDPS was highly similar to GGPPS11 from Arabidopsis thaliana. The quantitative real-time PCR showed that CsGGDPS expression was increased during the developmental process and increased with the age of tea leaves. Meanwhile, its expression was also enhanced during the Zuoqing procedure. CsGGDPS was ubiquitously expressed in the C. sinensis cv. Huangdan, cv. Tieguanyin and their first filial generation cv. Jinguanyin, but with different expression levels.

Key words: Camellia sinensis, GGDPS, tea aroma, gene expression

CLC Number:

YAO Xueqian, YUE Chuan, YANG Guoyi, CHEN Dan, ZHANG Dongtao, CHEN Guixin, YE Naixing. Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2017, 37(1): 86-96.

References 34

[1]	张正竹, 施兆鹏, 宛晓春. 萜类物质与茶叶香气(综述)[J]. 安徽农业大学学报, 2000, 27(1): 51-54.
[2]	吴勇. 萜烯类化合物与茶叶香气[J]. 化学工程与装备, 2009, 32(11): 123-125.
[3]	魏攀, 孟利军, 陈千思, 等. 烟草牻牛儿基牻牛儿基焦磷酸合成酶基因NtGGPPS1的克隆和功能分析[J]. 烟草科技, 2016, 49(4): 8-15.
[4]	闵丹丹, 唐美琼, 李刚, 等. 三七香叶基香叶基焦磷酸合酶基因的克隆及表达分析[J]. 中国中药杂志, 2015, 40(11): 2090-2095.
[5]	杨滢. 植物萜类代谢途径关键酶的比较及其在丹参中的表达分析[D]. 西安: 陕西师范大学, 2012: 1-2.
[6]	Beck G, Coman D, Herren E, et al.Characterization of the GGPP synthase gene family in Arabidopsis thaliana[J]. Plant Molecular Biology, 2013, 82(4): 393-416.
[7]	Yamamura Y, Mizuguchi Y, Taura F, et al.Transcriptional activation of a geranylgeranyl diphosphate synthase gene,GGPPS2,isolated from Scoparia dulcis by treatment with methyl jasmonate and yeast extract[J]. Journal of Natural Medicines, 2014, 68(4): 748-753.
[8]	李泽锋, 魏攀, 夏玉珍, 等. 烟草牻牛儿基牻牛儿基焦磷酸合成酶基因家族的全基因组鉴定[J]. 烟草科技, 2015, 48(6): 1-8.
[9]	张萌, 苏平, 刘雨佳, 等. 雷公藤牻牛儿基牻牛儿基焦磷酸合酶基因全长cDNA的获得及生物信息学分析[J]. 中国中药杂志, 2015, 40(6): 1066-1070.
[10]	韩立敏. 菘蓝牦牛儿基牦牛儿基焦磷酸合成酶基因 (IiGGPPS1) 的克隆及其表达特性分析[J]. 基因组学与应用生物学, 2015, 34( 6): 1172-1178.
[11]	Wei X, Yan X, Fu M W, et al.In silico analysis and feeding assays of some genes in the early steps of terpenoid biosynthetic pathway in Camellia sinensis[J]. Journal of Tea, 2013, 39(4): 191-198.
[12]	徐燕. 茶树萜类合成途径关键基因克隆及表达研究[D]. 杭州: 浙江大学, 2013: 12.
[13]	温立香, 郭雅玲, 黄寿辉. 乌龙茶做青技术的研究进展[J]. 安徽农业科学, 2015, 43(24): 215-217.
[14]	宛晓春. 茶叶生物化学[M]. 3版. 北京: 中国农业出版社, 2003: 119-120.
[15]	HEMMI H, NOIKE M, NAKAYAMA T, et al.An alternative mechanism of product chain-length determination in type III granylgeranyl diphosphate synthase[J]. Eur J Biochem, 2003, 270(10): 2186-2194.
[16]	Song L, Poulter C D.Yeast farnesyl-diphosphate synthase: site-directed mutagenesis of residues in highly conserved prenyltransferase domainsⅠand Ⅱ[J]. Proc Natl Acad Sci USA, 1994, 91(8): 3044-3048.
[17]	刘晶晶, 王富民, 刘国峰, 等. 茶树萜类香气物质代谢谱与相关基因表达谱时空变化的关系[J]. 园艺学报, 2014, 41(10): 2094-2106.
[18]	张冬桃. 乌龙茶做青过程香气形成相关基因的分离与表达[D]. 福州: 福建农林大学, 2016: 54.
[19]	Dudareva N, Pichersky E.Biology of floral scent [M]. Boca Raton: CRC Press, 2006: 55-78.
[20]	许宁, 陈宗懋, 游小清. 引诱茶尺蠖天敌寄生蜂的茶树挥发物的分离与鉴定[J]. 昆虫学报, 1999, 42(2): 126-131.
[21]	吴颖, 戴永峰, 张凌云. 做青工艺对乌龙茶品质影响研究进展[J]. 广东茶业, 2013, 31(5): 8-11.
[22]	张秀云, 方世辉, 夏涛. 乌龙茶萎凋做青中β-葡萄糖苷酶活性变化研究[J]. 安徽农业大学学报, 2000, 27(2): 164-166.
[23]	杨意成, 梁月荣. 乌龙茶花香形成机理的研究[J]. 茶叶, 2008, 34(1): 10-14.
[24]	梁晓岚, 陈春林. 乌龙茶香气形成机理初探[J]. 广东农业科学, 1996, 23(4): 23-25.
[25]	Yang Z Y, Baldermann S, Watanabe N.Recent studies of the volatile compounds in tea[J]. Food Research International, 2013, 53(2): 585-599.
[26]	黄福平, 陈荣冰, 梁月荣, 等. 乌龙茶做青过程中香气组成的动态变化及其与品质的关系[J]. 茶叶科学, 2003, 23(1): 31-37.
[27]	陈林, 陈键, 陈泉宾, 等. 做青工艺对乌龙茶香气组成化学模式的影响[J]. 茶叶科学, 2014, 34(4): 387-395.
[28]	王日为, 张丽霞, 杨伟丽, 等. 乌龙茶做青过程中香气的动态变化规律[J]. 湖南农业大学学报, 1999, 25(3): 194-199.
[29]	Lin J, Zhang P, Pan Z Q, et al.Discrimination of Oolong tea (Camellia sinensis) varieties based on feature extraction and selection from aromatic profiles analysed by HS-SPME/ GC-MS[J]. Food Chemistry, 2013, 141(1): 259-265.
[30]	许晨璐, 孙晓梅, 张守攻. 基因差异表达与杂种优势形成机制探讨[J]. 遗传, 2013, 35(6): 714-726.
[31]	Li X H, Wei Y L, Nettleton D, et al.Comparative gene expression profiles between heterotic and non-heterotic hybrids of tetraploid Medicago sativa[J]. BMC Plant Biol, 2009, 9(1): 107-115.
[32]	郭吉春, 叶乃兴, 杨如兴, 等. 茶树杂交种金观音、黄观音的选育与应用[J]. 福建茶叶, 2008, 31(1): 11-14.
[33]	王让剑, 杨军, 孔祥瑞, 等. 福建15个茶树品种SSR遗传差异分析与指纹图谱建立[J]. 福建农业学报, 2014, 29(10): 970-975.
[34]	盖钧镒, 章元明, 王建康. 植物数量性状遗传体系[M]. 北京: 科学出版社, 2003: 145-156.

Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant (Camellia sinensis)

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