茶树WRKY转录因子基因CsWRKY57的克隆及表达分析

Abstract

Abstract: The WRKY is one of the characteristic transcription factors in plants, which play important roles in plant growth, development and stress regulation. In order to study the relationship between WRKY transcription factors and stress tolerance of tea plant (Camellia sinensis), a WRKY transcription factor was cloned from tea cultivar ‘Shanchayihao’ and named CsWRKY57, based on the searching result of tea plant transcriptome database. Bioinformatics analysis showed that the full-length sequences of CsWRKY57 was 1β222βbp encoding 303 amino acids. The molecular weight of CsWRKY57 was 33.5βkD and theoretical isoelectric point was 5.49. The BLAST results showed that CsWRKY57 contained one typical WRKY domain and one zinc finger motif (C2H2), suggesting that it was a member of the WRKYIIc family. In addition, quantitative real-time PCR (qRT-PCR) analysis showed that the expression of CsWRKY57 was induced by salt, drought and ABA stresses, and showed a tendency to increase first and then decrease, which implies that CsWRKY57 is involved in the process of tea plant responses to salt, drought and ABA. Furthermore, transcriptional activation activity assays indicated that CsWRKY57 didn't have transcriptional activation activity, which means that CsWRKY57 may be needed to combine with other elements to activate gene expression.

Key words: tea plant (Camellia sinensis), CsWRKY57, cloning, expression analysis

CLC Number:

S571.1
Q52

GUO Junhong, WANG Weidong, GU Xing, GUO Shasha, GAO Yuefang, YANG Yajun, XIAO Bin. Cloning and Expression Analysis of WRKY Transcription Factor Gene CsWRKY57 in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2017, 37(4): 411-419.

References 25

[1]	Chi Y, Yang Y, Zhou Y, et al.Protein-protein interactions in the regulation of WRKY transcription factors[J]. Mol Plant, 2013, 6(2): 287-300.
[2]	Guo D, Zhang J, Wang X, et al.The WRKY transcription factor WRKY71/EXB1 controls shoot branching by transcriptionally regulating RAX genes in Arabidopsis[J]. Plant Cell, 2015, 27(11): 3112-3127.
[3]	Choi C, Hwang S H, Fang I R, et al.Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens[J]. New Phytologist, 2015, 208(3):846-859.
[4]	Wang X, Guo R, Tu M, et al.Ectopic expression of the wild grape WRKY transcription Factor VqWRKY52 in Arabidopsis thaliana enhances resistance to the biotrophic pathogen powdery mildew but not to the necrotrophic pathogen Botrytis cinerea[J]. Frontiers in Plant Science, 2017, 8: 97.
[5]	Wang H, Meng J, Peng X, et al.Rice WRKY4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani, the causing agent of rice sheath blight[J]. Plant Molecular Biology, 2015, 89(1): 157-171.
[6]	Raineri J, Wang S, Peleg Z, et al.The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress[J]. Plant Molecular Biology, 2015, 88(4): 401-413.
[7]	He G H, Xu J Y, Wang Y X, et al.Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis[J]. BMC Plant Biology, 2016, 16(1): 116-131.
[8]	Jaffar M A, Song A, Faheem M, et al.Involvement of CmWRKY10 in drought tolerance of chrysanthemum through the ABA-signaling pathway[J]. International Journal of Molecular Sciences, 2016, 17(5): 693-706.
[9]	Jiang Y, Yu D.The WRKY57 transcription factor affects the expression of jasmonate ZIM-Domain genes transcriptionally to compromise Botrytis cinerea resistance[J]. Plant Physiology, 2016, 171(4): 2771-2782.
[10]	Jiang Y, Liang G, Yang S, et al.Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence[J]. Plant Cell, 2014, 26(1): 230-245.
[11]	Jiang Y, Liang G, Yu D.Activated expression of WRKY57 confers drought tolerance in Arabidopsis[J]. Mol Plant, 2012, 5(6):1375-1388.
[12]	Jiang Y, Qiu Y, Hu Y, et al.Heterologous expression of AtWRKY57 confers drought tolerance in oryza sativa[J]. Frontiers in Plant Science, 2016, 7(145): 145.
[13]	Wang Y, Shu Z, Wang W, et al.CsWRKY2, a novel WRKY, gene from Camellia sinensis, is involved in cold and drought stress responses[J]. Biologia Plantarum, 2016, 60(3): 443-451.
[14]	杨桂燕, 贾彩霞, 孙宇栋, 等. 核桃JrsHSP17.3基因克隆及温度胁迫响应模式分析[J]. 西北植物学报, 2015, 35(9): 1752-1756.
[15]	钱文俊, 岳川, 曹红利, 等. 茶树中性/碱性转化酶基因CsINV10的克隆与表达分析[J]. 作物学报, 2016(3): 376-388.
[16]	Yamasaki K, Kigawa T, Seki M, et al.DNA-binding domains of plant-specific transcription factors: structure, function, and evolution[J]. Trends in Plant Science, 2013, 18(5): 267-76.
[17]	Tang L, Cai H, Zhai H, et al.Overexpression of Glycine soja WRKY20 enhances both drought and salt tolerance in transgenic alfalfa (Medicago sativa L.)[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2014, 118(1): 77-86.
[18]	Yan H, Jia H, Chen X, et al.The cotton WRKY transcription factor GhWRKY17 Functions in drought and salt stress in transgenic Nicotiana benthamiana through ABA signalling and the modulation of reactive oxygen species production[J]. Plant & Cell Physiology, 2014, 55(12): 2060-2076.
[19]	Liu L, Zhang Z, Dong J, et al.Overexpression of MtWRKY76, increases both salt and drought tolerance in Medicago truncatula[J]. Environmental & Experimental Botany, 2016, 123: 50-58.
[20]	Jiang Y Q, Deyholos M K.Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses[J]. Plant Molecular Biology, 2009, 69(1): 91-105.
[21]	Ying Z, Yu H, Yang X, et al.CsWRKY46, a WRKY transcription factor from cucumber, confers cold resistance in transgenic-plant by regulating a set of cold-stress responsive genes in an ABA-dependent manner[J]. Plant Physiology & Biochemistry Ppb, 2016, 108: 478-487.
[22]	Li P, Song A, Gao C, et al.The over-expression of a chrysanthemum WRKY transcription factor enhances aphid resistance[J]. Plant Physiology & Biochemistry, 2015, 95: 26-34.
[23]	Li P, Song A, Gao C, et al.Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants[J]. Plant Cell Reports, 2015, 34(8): 1365-1378.
[24]	Zhou Q Y, Tian A G, Zou H F, et al.Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, and, GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants[J]. Plant Biotechnol J, 2008, 6(5): 486-503.
[25]	王涛. 青蒿AaGL2基因的克隆及转基因青蒿遗传分析[D]. 上海: 上海交通大学, 2012.

Cloning and Expression Analysis of WRKY Transcription Factor Gene CsWRKY57 in Tea Plant (Camellia sinensis)

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