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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (4): 440-446.doi: 10.13305/j.cnki.jts.2019.04.009

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Determination of Pymetrozine in Tea Products by Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

YU Huan1,2, ZHOU Li1,*, LIN Qin1,2, YANG Jie1,2, SUN Hezhi1, WU Xudong3, WANG Xinru1, ZHANG Xinzhong1, CHEN Zongmao1, LUO Fengjian1,*   

  1. 1. Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008,China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Pu'er Comprehensive Technical Testing Center, Pu'er 665099, China
  • Received:2018-12-13 Online:2019-08-15 Published:2019-08-19

Abstract: An analytical method for the determination of pymetrozine residues in tea products, including dry tea, matcha and instant tea powder, was established based on Cleanert PCX solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The residual pymetrozine in dry tea, matcha and instant tea powder was extracted by a mixture of methanol and water, cleaned up and concentrated by a Cleanert PCX SPE column, and separated on an Acquity BEH C18 column. Pymetrozine level was detected by UPLC-MS/MS on multiple reaction monitoring (MRM) mode and quantified with external standard method. In the spiked range of 0.005-1.000 mg·kg-1, the average recovery of pymetrozine was 77.0%-95.1%, and the relative standard deviation (RSD, n=5) ranged from 1.8%-6.9%. The limit of quantitation (LOQ) was 0.005 to 0.010 mg·kg-1. The sensitivity, precision and recovery of the developed method meet the requirements for residual pesticides analysis in various tea products. The results of this study can provide an analytical method for the determination and risk assessment of pymetrozine in tea products.

Key words: pymetrozine, tea, pesticide residue, solid phase extraction, UPLC-MS/MS

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