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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (6): 589-594.doi: 10.13305/j.cnki.jts.2018.06.005

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Determination of 2,4-Epibrassinolide in Different Teas by Ultra Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry with Dispersive Solid Phase Extraction

ZHU Li, CHEN Hongping, CHAI Yunfeng, MA Guicen, HAO Zhenxia, WANG Chen, LIU Xin*, LU Chengyin*   

  1. Tea Quality and Supervision Testing Center, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment for Tea, Ministry of Agriculture and Rural Affairs (Hangzhou), Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China
  • Received:2018-04-28 Revised:2018-08-03 Online:2018-12-15 Published:2019-12-15

Abstract: An efficient method for analyzing 2,4-Epibrassinolide in different teas was developed based on ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Homogenized samples were extracted with acetonitrile solution, cleaned up with three sorbents including C18, strong anion exchanger (SAX) and graphitized carbon black (GCB). The compound was separated on a HSS T3 column using electrospray Ionization (ESI) mode, detected by scheduled multiple reaction monitoring (SMRM) and quantified by matrix-matched external standard curves. The compound showed good linearity between the concentration of 0.8-800βμg·L-1, with high significant correlation (R2>0.999). The limit of quantitation (LOQ, S/N=10) ranged from 0.55 to 1.46βμg·kg-1. In addition, the spiked (20, 40, 200βμg·kg-1) recoveries of tea samples were 75.5%-93.6%, and RSDs were 0.4%-7.0%. The method was accurately and sensitively applied to commercial tea samples, which fulfilled all requirement for the determination of 2,4-Epibrassinolide in tea samples.

Key words: tea, 2, 4-Epibrassinolide, brassinolide, brassinosteroids, ultra performance liquid chromatography- tandem mass spectrometry, dispersive solid phase extraction

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