SNaPshot技术检测茶树SNP研究

doi:10.13305/j.cnki.jts.2014.02.014

Abstract

Abstract: In order to increase the genotyping efficiency of tea SNPs and promote the application of SNPs in tea genetic breeding investigation, the feasibility of SNaPshot in SNPs genotyping of tea plant was investigated. Ten SNPs were selected from previous experiment as target SNPs. Then, these SNPs were detected in different tea cultivars by SNaPshot technology. Six among 10 SNPs were successful detected, with an accuracy of 60%. The polymorphism diversity of these SNPs was also analyzed. The value of N_A was 2, H_e ranged from 0.37 to 0.52, H_o ranged from 0.32 to 0.74, PIC ranged from 0.36 to 0.50. All these markers were shown coincide between `Longjing43` and its repeated test. The markers reported here will be useful for tea genetic linkage map construction and genetic diversity study. The SNaPshot technology will promote the genetic study and accelerate the breeding process in tea plant.

Key words: tea plant, SNPs, SNaPshot, genetic diversity, genetic linkage map

CLC Number:

S571.1
Q52

ZHANG Chengcai, TAN Liqiang, WANG Liyuan, WEI Kang, CHENG Hao. Study of SNaPshot Detect SNP Markers in Tea Plant[J]. Journal of Tea Science, 2014, 34(2): 180-187.

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Study of SNaPshot Detect SNP Markers in Tea Plant

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