氮素水平对茶树新梢叶片代谢谱及其昼夜变化的影响

doi:10.13305/j.cnki.jts.2013.06.008

Abstract

Abstract: Amino acids, polyphenols, caffeine and other metabolites such as sugars and aromatic substances are important determinants of tea quality. Different cultivation practices affect tea quality by altering the basic metabolite profiles. In this study, the metabolomic changes through nitrogen and samples collected in day and night were investigated in fresh tea shoots by ¹H-NMR for non-targeted analysis coupled with a multivariate data analysis and by HPLC for targeted analysis to reveal the mechanism of formation, transformation and relationship of the major characteristic quality components in fresh tea leaves. Non-targeted analysis with ¹H-NMR showed significant effect of samples collected in day and night but no difference among N application rates. However, targeted analysis with HPLC showed both significant variations in amino acids and catechins as affected by N rates and light/dark change. There were higher sensitivity of amino acids and sugars but lower sensitivity of tea polyphenols through the metabolomic analysis using ¹H-NMR.

Key words: Camellia sinensis, nitrogen application rate, light/dark, polyphenols, amino acids

CLC Number:

YANG Yi-yang, MA Li-feng, LI Xing-hui, R. G. Ratcliffe, N. J. Kruger, RUAN Jian-yun. Metabonomic Analysis on the Effects of different Nitrogen Application Rates and Light/Dark Change on Metabolism in Tea Leaves[J]. Journal of Tea Science, 2013, 33(6): 491-499.

References 50

[1]	Nicholas W, Coralyn W, David A, et al. The analgesic effects of caffeine in headache[J]. Pain, 1991, 44: 151-155.
[2]	Yang C S, Wang X, Lu G, et al. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature Reviews Cancer, 2009, 9: 429-439.
[3]	Ritsner M S, Miodownik C, Ratner Y, et al. L-Theanine relieves positive, activation, and anxiety symptoms in patients with schizophrenia and schizoaffective disorder: An 8-week, randomized, double-blind, placebo-controlled, 2-center study[J]. The Journal of clinical psychiatry, 2011, 1(72): 34-42.
[4]	Okano K, Chutani K, Matsuo K.Suitable level of nitrogen fertilizer for tea (Camellia sinensis L.) plants in relation to growth, photosynthesis, nitrogen uptake and accumulation of free amino acids[J]. Japanese Journal of Crop Science, 1997, 66(2): 279-287.
[5]	Ruan J Y, Gerendás J, Hardter R, et al. Effect of root zone pH and form and concentration of nitrogen on accumulation of quality-related components in green tea[J]. Journal of the Science of Food and Agriculture, 2007b, 87: 1505-1516.
[6]	伍炳华, 韩文炎, 姚国坤. 茶树氮磷钾营养的品种间差异[J]. 茶叶科学, 1991, 11(1): 11-18.
[7]	苏有健, 廖万友, 丁勇, 等. 不同氮营养水平对茶叶产量和品质的影响[J]. 植物营养与肥料学报, 2011, 17(6): 1430-1436.
[8]	Chu D C.Green tea: Its cultivation, processing of the leaves for drinking materials and kinds of green tea[M]//Chemistry and Applications of Green Tea. Yamamoto T Juneja R L, Chu D C, Kim M. CRC Press: Boca Raton, F L.1997: 1-11.
[9]	Takeuchi A, Matsumoto S, Hayatsu M.Effects of shading treatment on the expression of the genes for chalcone synthase and phenylalanine ammonia-lyase in tea plant (Camellia sinensis)[C]//National Research Institute of Vegetables. Ornamental Plants and Tea. Series B. 1995, 8: 1-9.
[10]	黄雨初, 汪东风, 陈为均, 等. 光对茶树儿茶素代谢的影响[J]. 应用生态学报, 1995, 6(2): 220-222.
[11]	潘根生, 高人俊. 茶树遮荫生理生化变化[J]. 茶叶科学, 1986, 6(2): 1-6.
[12]	Ma C F, Wang H H, Lu X, et al. Analysis of Artemisia annua L. volatile oil by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry[J]. Journal of Chromatography A, 2007, 1150: 50-53.
[13]	Fiehn O.Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks[J]. Comparative and Functional Genomics, 2001, 2: 155-168.
[14]	Borse B B, Rao L J M, Nagalakshmi S, et al. Fingerprint of black teas from India: identification of the regio-specific characteristics[J]. Food Chemistry, 2002, 79: 419-424.
[15]	Fernández P L, Pablos F, Martin M J, et al. Study of catechin and xanthine tea profiles as geographical tracers[J]. Journal of Agricultural and Food Chemistry, 2002, 50: 1833-1839.
[16]	陈波, 张巍, 康海宁, 等. 茶叶的1H-NMR指纹图谱研究[J]. 波谱学杂志, 2006, 23: 169-180.
[17]	陈全胜, 赵杰文, 张海东, 等. SIMCA模式识别方法在近红外光谱识别茶叶中的应用[J]. 食品科学, 2006, 27: 186-189.
[18]	Li X L, He Y, Wu C Q, et al. Nondestructive measurement and fingerprint analysis of soluble solid content of tea soft drink based on Vis/NIR spectroscopy[J]. Journal of Food Engineering, 2007, 82: 316-323.
[19]	Dorsten F A V, Daykin C A, Mulder T P J, et al. Metabonomics approach to determine metabolic differences between green tea and black tea consumption[J]. Journal of Agricultural and Food Chemistry, 2006, 54: 6929-6938.
[20]	Ohno A, Oka K, Sakuma C, et al. Characterization of tea cultivated at four different altitudes using 1H NMR analysis coupled with multivariate statistics[J]. Journal of Agricultural and Food Chemistry, 2011, 59(10): 5181-5187.
[21]	Lee J E, Lee B J, Hwang J A, et al. Metabolic dependence of green tea on plucking positions revisited: A metabolomic study[J]. Journal of Agricultural and Food Chemistry, 2011, 59(19): 10579-10585.
[22]	Nicholson J K, Wilson I D.Understanding ‘global’ systems biology: Metabonomics and the continuum of metabolism[J]. Nature Reviews Drug Discovery, 2003, 2: 668-676.
[23]	Ward J L, Harrisa C, Lewisa J, et al. Assessment of 1H-NMR spectroscopy and multivariate analysis as a technique for metabolite fingerprinting of Arabidopsis thaliana[J]. Phytochemistry, 2003, 62: 949-957.
[24]	Nicholson J K, Connelly J, Lindon J C, et al. Metabonomics: a platform for studying drug toxicity and green function[J]. Nature Reviews Drug Discovery, 2002, 1: 153-161.
[25]	Bamforth F J, Dorian V, Vallance H, et al. Diagnosis of inborn errors of metabolism using 1H NMR spectroscopic analysis of urine[J]. Journal of Inherited Metabolic Disease, 1999, 22: 297-301.
[26]	Griffin J L, Walker L A, Garrod S, et al. NMR spectroscopy based metabonomic studies on the comparative biochemistry of the kidney and urine of the bank vole(Clethrionomys galreolus), wood mouse(Apodemus sylvaticus), white toothed shrew(Crocidura suavolens) and the laboratory rat[J]. Comparative Biochemistry and Physiology Part B: Biochemistry Molecular Biology, 2000, 127: 357-367.
[27]	Bundy J G, Osborn D, Weeks J M, et al. An NMR based metabonomic approach to the investigation of coelomic fluid biochemistry in earthworms under toxic stress[J]. FEBS Letters, 2001, 500: 331-335.
[28]	Aranibar N, Singh B K, Stockton G W, et al. Automated mode-of-action detection by metabolic profiling[J]. Biochemical and Biophysical Research Communications, 2001, 286: 150-155.
[29]	Bailey N J C, Oven M, Holmes E, et al. Metabolomic analysis of the consequences of cadmium exposure in Silene cucubalus cell cultures via 1H NMR spectroscopy and chemometrics[J]. Phytochemistry, 2003, 62: 851-858.
[30]	Ghauri F Y, Blackledge C A, Glen R C, et al. Quantitative structure-metabolism relationships for substituted benzoic acids in the rat: Computational chemistry, NMR spectroscopy and pattern recognition studies[J]. Biochemical Pharmacology, 1992, 44: 1935-1946.
[31]	Kessler H.Structure-activity relationships by NMR: A new procedure for drug discovery by a combinatorial-rational approach[J]. Angewandte chemie international edition in English, 1997, 36: 829-831.
[32]	Hammond D G, Kubo I.Structure-activity relationship of alkanols as mosquito larvicides with novel findings regarding their mode of action[J]. Bioorganic & Medicinal Chemistry, 1999, 7: 271-278.
[33]	Bailey N J C. Imperial college of science technology and medicine[D]. London: University of London, 2000.
[34]	Kruger N J, Huddleston J E, Le Lay P, et al. Network flux analysis: impact of 13C-substrates on metabolism in Arabidopsis thaliana cell suspension cultures[J]. Phytochemistry, 2007, 68: 2176-2188.
[35]	Del Rio D, Stewart A J, Mullen W, et al. Brighenti F, Crozier A. HPLC-MSn analysis of phenolic compounds and purine alkaloids in green and black tea[J]. Journal of Agricultural and Food Chemistry, 2004, 52(10): 2807-2815.
[36]	成浩, 王丽鸳, 周健, 等. 基于化学指纹图谱的绿茶原料品种判别分析[J]. 中国农业科学, 2008, 41: 2413-2418.
[37]	高向阳,刘娜. AccQ·Tag柱前衍生反相-高效液相色潽法测定齿果酸模中的氨基酸含量[J].食品科学, 2011, 32(20): 160-163.
[38]	Fujiwara M, Ando I, Arifuku K.Multivariate analysis for 1H-NMR spectra of two hundred kinds of tea in the world[J]. Analytical Science: the international journal of the Japan society for analytical chemistry, 2006, 22: 1307-1314.
[39]	Tarachiwin L, Ute K, Koba yashi A, et al. 1H NMR based metabolic profiling in the evaluation of Japanese green tea quality[J]. Journal of Agricultural and Food Chemistry, 2007, 55: 9330-9336.
[40]	Ohta K, Harada K.Studies on environmental conditions of tea plants cultivated by hydroponics: effects of irradiation and night temperature on free amino acids contents and plant growth[J]. Environment Control in Biology, 1996, 34: 179-190.
[41]	Ku K M, Choi J N, Kim J, et al. Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia sinensis L.)[J]. Journal of Agricultural and Food Chemistry, 2010, 58: 418-426.
[42]	Lee J E, Lee B J, Chung J O, et al. Geographical and climatic dependencies of green tea (Camellia sinensis) metabolites: A 1H NMR-based metabolomics study[J]. Journal of Agricultural and Food Chemistry, 2010, 58: 10582-10589.
[43]	Mukai T, Horie H, Goto T.Differences in free amino acids and total nitrogen contents among various prices of green teas[J]. Journal of Tea Research, 1992, 76: 45-50.
[44]	Wang K B, Ruan J Y.Analysis of chemical components in green tea in relation with perceived quality, a case study with Longjing teas[J]. International Journal of Food Science & Technology, 2009, 44: 2476-2484.
[45]	Cloughley J B.Effects of harvesting policy and nitrogen application rates on the production of tea in Central Africa. II. Quality and total value of the crop[J]. Experimental Agriculture, 1983, 19: 47-54.
[46]	Kamau D M, Spiertz J H J, Oenema O, et al. Productivity and nitrogen use of tea plantations in relation to age and genotype[J]. Field Crops Research, 2008, 108: 60-70.
[47]	Owuor P O, Odhiambo H O.Response of some black tea quality parameters to nitrogen fertiliser rates and plucking frequencies[J]. Journal of the Science of Food and Agriculture, 1994, 66: 555-561.
[48]	Han W Y, Ma L F, Shi Y Z, et al. Nitrogen release dynamics and transformation of slow release fertiliser products and their effects on tea yield and quality[J]. Journal of the Science of Food and Agriculture, 2008, 88: 839-846.
[49]	Ruan J Y, Gerendás J, Hardter R, et al. Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants[J]. Annals of Botany, 2007a, 99: 301-310.
[50]	Fiehn O.Metabolomics: the link between genotype and phenotypes[J]. Plant Molecular Biology, 2002, 48: 155-171.

[1]	. [J]. Journal of Tea Science, 1965, 2(04): 59 -63 .
[2]	. [J]. Journal of Tea Science, 1965, 2(03): 18 -21 .
[3]	. [J]. Journal of Tea Science, 1965, 2(03): 22 -24 .
[4]	. [J]. Journal of Tea Science, 1966, 3(02): 55 -57 .
[5]	. [J]. Journal of Tea Science, 1966, 3(02): 82 -83 .
[6]	. [J]. Journal of Tea Science, 1986, 6(01): 1 -8 .
[7]	. [J]. Journal of Tea Science, 1987, 7(02): 29 -34 .
[8]	. [J]. Journal of Tea Science, 1988, 8(01): 37 -42 .
[9]	. [J]. Journal of Tea Science, 1995, 15(02): 154 .
[10]	. [J]. Journal of Tea Science, 1999, 19(01): 73 -76 .

Metabonomic Analysis on the Effects of different Nitrogen Application Rates and Light/Dark Change on Metabolism in Tea Leaves

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