茶树α-tubulin基因的原核表达及其多克隆抗体的制备

doi:10.13305/j.cnki.jts.2009.5.002

Abstract

Abstract: According to the relevant complete mRNA sequence of α-tubulin gene of Camellia sinensis (accession NO. DQ340766), the coding region of α-tubulin gene was amplified using RT-PCR method, and its products were expressed in Escherichia coli using the pET-32a(+) vector. The fusion protein was expressed in the form of inclusion body when induced with isopropyl-D-thiogalatopyranoside (IPTG), then was purified to immunize the rabbit to produce the polyclonal antibody against α-tubulin. The specificity of the antibody was confirmed by Western blot analysis. The results of Western blot revealed well specificity of the antibody against α-tubulin in Camellia sinensis plant.

Key words: tea plant(Camellia sinensis), α-tubulin, prokaryotic expression, polyclonal antibody

CLC Number:

S571
Q785

TAN Zhen, TONG Xin, FANG Chao, JIANG Chang-jun, CHEN Cong. Prokaryotic Expression of α-tubulin Gene of Camellia sinensis and Preparation of α-tubulin Polyclonal Antibody[J]. Journal of Tea Science, 2009, 29(5): 336-340.

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Prokaryotic Expression of α-tubulin Gene of Camellia sinensis and Preparation of α-tubulin Polyclonal Antibody

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