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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (5): 576-586.doi: 10.13305/j.cnki.jts.2019.05.009

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Determination of 79 Pesticide Residues in Tea by Solid Phase Extraction with GC-MS/MS and LC-MS/MS

ZENG Yan1, LANG Hong1, YANG Qiaohui1, 2, LI Xiaxue1, SHI Chaoting1   

  1. 1. Ya'an Quality Inspection of Agricultural Products Monitoring Center, Ya'an 625000, China;
    2. College of Food Science, Sichuan Agricultural University, Ya'an 625000, China
  • Received:2019-03-01 Revised:2019-04-24 Online:2019-10-15 Published:2019-10-15

Abstract: In order to detect various pesticide residues in tea, a reliable, rapid and high throughput detection method was established: a solid phase extraction was developed for the determination of 79 pesticide residues in tea by gas chromatography-tandem mass spectrometry (GC-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). These samples were extracted by 20βmL acetonitrile. 10βmL extracts were cleaned by Carbon/NH2 column with 20βmL acetonitrile + toluene (3∶1) and then mixed with 5βmL acetonitrile, then these procdssed samples were detected by GC-MS/MS and LC-MS/MS, respectively. The results show that the concentrations of 79 pesticide residues had good linearity relationships in the range of 0.01-0.40βmg·L-1, with the correlation coefficients (R2) above 0.995, the recoveries of high, low and medium levels were ranged from 67.3%-130.8%, the relative standard deviations (RSDs) were less than 15%, the limits of quantification (LOQs) of 98.7 % pesticides were less than or equal to 0.01βmg·kg-1, multiple types of tea were detected, and the results were proved to be qualified. Consequently, the method can simultaneously detect 5 kinds of pesticides, including organophosphorus, organochlorine, pyrethroids, carbamates and organic heterocyclic pesticides, which would be useful to determine multi-pesticide residues in high numbers of tea samples.

Key words: tea, GC-MS/MS, LC-MS/MS, pesticide residues

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