Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (1): 39-50.doi: 10.13305/j.cnki.jts.20200117.007

Previous Articles     Next Articles

Cloning and Expression Analysis of CsPPH Gene in Tea Plant (Camellia sinensis)

ZHOU Zhe1,2,4, CHEN Zhidan2,3,4, WU Quanjin5, XU Yilan3, SUN Weijiang1,2,3,4,*   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Collaborative Innovation Center of Modern Agriculture Industrial Park of Anxi County, Quanzhou 362400, China;
    3. College of Tea, Fujian Agriculture and Forestry University, Quanzhou 362400, China;
    4. Engineering Technology and Research Center of Fujian Tea Industry, Fuzhou 350002, China;
    5. Department of Finance and Management, The Open University of Fujian, Fuzhou 350002, China
  • Received:2019-06-10 Revised:2019-09-02 Online:2020-02-15 Published:2020-02-04

Abstract: Pheophytinase (PPH) is a key enzyme in chlorophyll degradation. It can convert pheophytin a into pheophorbide a, which is the last product to keep green in chlorophyll degradation pathway. This step is considered to be a key step of leaf senescence and yellowing. In this study, the full-length sequence of CsPPH gene was cloned from the new shoot leaves of albino tea plant Camellia sinensis cv. Baijiguan (MK359094), and its biological characteristics were analyzed. The full-length of CsPPH was 1 298 bp, and the ORF was 1 241 bp, encoding 413 amino acids. Sequence analysis indicated that the encoded protein was a stable hydrophobic protein, and its molecular weight was predicted to be 45 741.50 Da. Its theoretical isoelectric point was 6.12. It was mainly located in chloroplasts. The real-time fluorescence quantitative PCR showed that under shading conditions, the expression of CsPPH was inhibited, chlorophyll increased and leaf color turned green. Light promoted the expression of CsPPH in leaves of cv. Baijiguan, and chlorophyll degradation led to leaf albinism.

Key words: albinism of tea plant, PPH gene, clone, bioinformatics analysis

CLC Number: