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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (3): 290-298.

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Research on the Protective Effects of TFDG on IL-1β-induced Inflammatory Injury in Rat Chondrocytes in Vitro

ZHOU Ying1, HUANG Qian2, ZHANG Tian1, CHEN Ping1,*   

  1. 1. Department of Tea Science, Zhejiang University, Hangzhou 310058, China;
    2. School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China
  • Received:2016-10-12 Revised:2017-01-05 Online:2017-06-15 Published:2019-08-22

Abstract: The chondrocytes were isolated and harvested from knee articular surface of male Sprague-Dawley rats, which were identified by toluidine blue and type II collagen immunofluorescence staining. To investigate potential protective effects of theaflavin-3,3′-digallate (TFDG) on IL-1β-induced inflammatory injury in rat chondrocytes in vitro, a model of osteoarthritis was established through stimulating rat chondrocytes with IL-1β. Inverted phase contrast microscopy showed TFDG treatment significantly improved osteoarthritis chondrocytes morphology. Real-time PCR results showed that TFDG treatment not only up-regulated chondrocyte marker Col II mRNA expression, but also down-regulated pro-inflammatory cytokines IL-1β and IL-6 mRNA expression. ELISA analysis further confirmed TFDG treatment significantly decreased the secretion of inflammatory factors. Western blot results showed that TFDG treatment significantly inhibited the inflammation-related enzyme COX-2 expression. Taken together, these results indicate that TFDG has a preventive effect on IL-1β-induced inflammatory injury in rat chondrocytes in vitro by reducing inflammatory reaction.

Key words: TFDG, osteoarthritis, chondrocytes, inflammation

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