儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响

doi:10.13305/j.cnki.jts.2003.02.007

茶叶科学 ›› 2003, Vol. 23 ›› Issue (2): 119-123.doi: 10.13305/j.cnki.jts.2003.02.007

儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响

朴宰日¹, 王岳飞¹, 杨贤强¹, 陈留记¹, 杨勇¹, 赵保路², 焦鸿利², 周淡宜³

1. 浙江大学茶学系,浙江杭州 310029;
2. 中国科学院生物物理研究所,北京 100010;
3. 浙江省医学科学院,浙江杭州 310013

收稿日期:2002-11-19 修回日期:2003-05-15 出版日期:2003-11-25 发布日期:2019-09-16
作者简介:朴宰日（1970- ）,男,韩国人,博士,主要从事茶叶生化研究。

Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice

PIAO Zai-ri¹, Wang Yue-fei¹, YANG Xian-qiang¹, CHEN Liu-ji¹, YANG Yong¹, ZHAO Bao-lu², JIAO Hong-li², ZHOU Dang-yi³

1.Department of Tea Science, Zhejiang University, Hangzhou,310029, China;
2. Institute of Biophysics, Academia Sinica1, Beijing 100101, China;
3. Zhejiang Academy of Medical Sciences, Hangzhou 310013, China

Received:2002-11-19 Revised:2003-05-15 Online:2003-11-25 Published:2019-09-16

摘要/Abstract

摘要： 观察茶儿茶素对铅诱导HepG2细胞氧化损伤和茶多酚对铅染毒小鼠体内铅含量的影响。采用MTT（四甲基偶氮唑盐）法检测细胞活力,TBA（硫代巴比妥酸）反应生成MDA（丙二醛）的量测量细胞脂质过氧化水平。以醋酸铅染毒小鼠,采用原子荧光光度法测定小鼠器官中的铅含量。结果发现儿茶素能改善铅诱导细胞的氧化损伤,使细胞活力升高,MDA下降;铅染毒后,小鼠血液、肝、肾、脑和股骨蓄积的铅含量显著增加,茶多酚对小鼠肝脏排铅有显著作用,但对其他器官的铅排泄无明显影响。

关键词: 茶学, 茶儿茶素, 茶多酚, 铅毒, 脂质过氧化, 排铅

Abstract: The effects of tea catechins on lead-induced lipid peroxidation damage in HepG2 cells and tea polyhenol on the content of lead in lead-exposed mice were investigated.HepG2 cell viability was determined using the MTT method,lipid peroxidation was assayed by detetmining the production rate of thibarbiturie acid reactive substance(TBARS)which was expressed as malondialdehyde(MDA)equivalents.The content of lead in lead-exposed mice was determined using hydride atomic fluorescence spectroscopic method. Results showed that tea catechins had protective effects on lead-exposed HepG2 cell viability and inhibitory effects on TBARS formation in HepG2 cells.All the indices of the mice in the exposed groups were significantly higher than those in the control.Tea polyphenol was able to accelerate the removal of lead from liver,but the same effects were not found in blood、kidney, brain and thigbones.

Key words: Tea science, Tea catechins, Tea polyphenol, Lead exposure, Lipid preoxidation, Removal of lead

中图分类号:

R963

朴宰日, 王岳飞, 杨贤强, 陈留记, 杨勇, 赵保路, 焦鸿利, 周淡宜. 儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响[J]. 茶叶科学, 2003, 23(2): 119-123. doi: 10.13305/j.cnki.jts.2003.02.007.

PIAO Zai-ri, Wang Yue-fei, YANG Xian-qiang, CHEN Liu-ji, YANG Yong, ZHAO Bao-lu, JIAO Hong-li, ZHOU Dang-yi. Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice[J]. Journal of Tea Science, 2003, 23(2): 119-123. doi: 10.13305/j.cnki.jts.2003.02.007.

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儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响

Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice

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