茶树内生防病和农药降解菌的分离

洪永聪, 辛伟, 来玉宾, 翁昕, 胡方平

茶叶科学 ›› 2005, Vol. 25 ›› Issue (3) : 183-188.

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茶叶科学 ›› 2005, Vol. 25 ›› Issue (3) : 183-188. DOI: 10.13305/j.cnki.jts.2005.03.005

茶树内生防病和农药降解菌的分离

  • 洪永聪1, 辛伟2, 来玉宾1, 翁昕1, 胡方平1, *
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Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant

  • HONG Yong-cong1, XIN Wei2, LAI Yu-bin1, WENG Xin1, HU Fang-ping1, *
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摘要

对不同茶树品种的健叶和病叶上分离的内生细菌进行了筛选和鉴定。结果表明,茶树体内存在大量的内生细菌,各品种间内生细菌的数量为2.9×106~39.4×106βcfu/(g•fw)。内生细菌的生物功能测定结果表明,菌株TL2的拮抗能力强,先接种菌株TL2 24βh后再接种茶轮斑病菌的防病效果好;同时菌株TL2对氯氰菊酯也表现出较强的降解能力;另外菌株TL2能在茶树上内生定殖。经鉴定,菌株TL2为枯草芽孢杆菌(Bacillus subtilis)。

Abstract

The screening and identification of endophytic bacteria from the healthy and diseased leaves of various tea cultivar plants were conducted. Results showed that the endophytic bacteria were accounted in 2.9×106~39.4×106βcfu/(g•fw) in tea cultivars plants. The TL2 stain showed strongly antagonistic activity against leaf fungal pathogens of tea-plant. It showed a good protecting effect when inoculated the TL2 strain bacteria firstly than inoculated the tea grey blight pathogen(Pestallozzia theae) later, Besides, it showed a strong degrative activity to cypermethrin. The TL2 bacteria strain could colonize within tea plant. According to the results of identification, the TL2 bacteria strain was identified as bacillas subtilis.

关键词

茶树 / 防病 / 枯草芽孢杆菌 / 内生细菌 / 农药降解

Key words

Antifungal / Bacillus subtilis / Endophytic Bacteria / Pesticide Degrading / Tea-plant

引用本文

导出引用
洪永聪, 辛伟, 来玉宾, 翁昕, 胡方平. 茶树内生防病和农药降解菌的分离[J]. 茶叶科学. 2005, 25(3): 183-188 https://doi.org/10.13305/j.cnki.jts.2005.03.005
HONG Yong-cong, XIN Wei, LAI Yu-bin, WENG Xin, HU Fang-ping. Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant[J]. Journal of Tea Science. 2005, 25(3): 183-188 https://doi.org/10.13305/j.cnki.jts.2005.03.005
中图分类号: Q939.129    S435.711   

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基金

国家自然科学基金资助项目(No.30370968)

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