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茶叶科学 ›› 2006, Vol. 26 ›› Issue (2): 141-146.doi: 10.13305/j.cnki.jts.2006.02.013

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茶叶中硫丹及硫丹硫酸酯的ELISA快速测定

吕潇1, 2, 任凤山2, 王文博2, 杜红霞2, 李慧冬2, 丁蕊艳2, 朱端卫1, LEENanjuA3, SHEILDRoss4, KENNEDYIvanR5   

  1. 1. 华中农业大学资源与环境学院,湖北 武汉 430070;
    2. 山东省农业科学院中心实验室,山东 济南 250100;
    3. Department of Food Science & Technology, University of NSW, Sydney, NSW, 2050, Australia;
    4. Agrisearch Analytical, Sydney NSW 2039, Australia;
    5. Faculty of Agriculture, Food and Natural Resources, University of Sydney, Sydney, NSW, 2006, Australia
  • 收稿日期:2005-12-12 修回日期:2006-01-12 出版日期:2006-06-25 发布日期:2019-09-10
  • 作者简介:吕潇(1957- ),女,研究员,在读博士生,主要从事农药残留分析与环境行为研究。
  • 基金资助:
    国家留学基金2002

Determination of Endosulfan and Endosulfan Sulfate in Tea by ELISA

LU Xiao1, 2, REN Feng-shan2, WANG Wen-bo2, DU Hong-xia2, LI Hui-dong2, DING Rui-yan2, ZHU Duan-wei1, LEE Nanju A3, SHEILD Ross4, KENNEDY Ivan R5   

  1. 1. Faculty of Natural Resources & Environment, Huazhong Agricultural University, Wuhan 430070, China;
    2. Central Laboratory, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China;
    3. Department of Food Science & Technology, University of NSW, Sydney, NSW, 2050, Australia;
    4. Agrisearch Analytical, Sydney, NSW, 2039, Australia;
    5. Faculty of Agriculture, Food and Natural Resources, University of Sydney, Sydney, NSW, 2006, Australia
  • Received:2005-12-12 Revised:2006-01-12 Online:2006-06-25 Published:2019-09-10

摘要: 研究应用酶联免疫方法,快速检测茶叶中硫丹及硫丹硫酸酯。茶叶提取后,不净化,用PBS缓冲液稀释500倍后,进行酶联免疫方法测定,方法平均回收率为86.6%~91.1%;经SPE净化后,回收率68.7%~123%。标准曲线IC50为2βµg/L,线性范围1~10βµg/L。直接提取法最低检测限2βmg/kg,净化法为0.06βmg/kg。经气相色谱/质谱联用方法验证,灵敏度、精密度和准确度均符合快速检测要求。该法具有快速、简便、低耗、易操作等特点。

关键词: 硫丹, 酶联免疫, 茶叶, 气相色谱/质谱联用仪

Abstract: A rapid method based on Enzyme-linked immunosorbent assay (ELISA) was developed for determination of endosulfan isomers and endosulfan sulfate in commercial black and green tea. The average recoveries obtained from crude extracts ranged from 86.6% to 91.1%, which from purified extracts ranged from 68.7% to 123%. The liner range of the standard curve was from 1βµg/L to 10βµg/L. The detection limit of crude extract was 2βmg/kg and the purified was 0.06βmg/kg. This study showed a validation of endosulfan analyzed in tea by ELISA and GC-MS at different levels and proved that ELISA is a quick, low cost and practical technique for monitoring pesticides in tea.

Key words: endosulfan, ELISA, tea, GC/MS

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