毛细管电泳分析茶黄素的方法研究

夏文娟; 张丽霞; 史作安; 贾明

doi:10.13305/j.cnki.jts.2006.02.014

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茶叶科学 ›› 2006, Vol. 26 ›› Issue (2) : 147-153. DOI: 10.13305/j.cnki.jts.2006.02.014

毛细管电泳分析茶黄素的方法研究

夏文娟, 张丽霞*, 史作安, 贾明

作者信息 +

Analysis of Theaflavins by Capillary Electrophoresis

XIA Wen-juan, ZHANG Li-xia*, SHI Zuo-an, JIA Ming

Author information +

文章历史 +

摘要

研究了毛细管电泳分析茶黄素类组分的最佳条件。结果表明：在250βmmol/L H₃BO₄、10βmmol/L KH₂PO₄、0.75βmmol/L SDS和20％(v/v)乙腈组成的pH7.5电泳介质中,选择25βkV电压、20℃柱温和200βnm波长检测,可在10βmin以内将茶叶中的四种主要茶黄素单体分离,并获得较高的检测灵敏度。四种茶黄素单体浓度与峰面积值之间的相关系数r=0.9945~0.9990,检测下限在0.53~0.64βμg/ml之间,加标回收率为92.50%~108.36%,变异系数≤2.67%。本方法快速、准确,可用于实际样品的测定。

Abstract

The investigation optimum analytical conditions in the determination of theaflavins(TFs) with capillary electrophoresis was described. The electrolyte solution was consisted of 250βmmol/L H₃BO₄, 10βmmol/L KH₂PO₄, 0.75βmmol/L SDS and 20% (v/v) acetonitrile under pH7.5. The work parameters of the instrument were selected as follows: voltage 25βkV, column temperature 20℃, and detecting wavelength 200βnm. By using this method, the four majors of TF could be separated each other completely within 10 minutes and each had a good linear relationship between its peak area and corresponding concentration (r=0.9945~0.9990). The minimum concentration limit was between 0.53~0.64βμg/ml. The mean recoveries of TFs monomers varied from 92.50% to 108.36%. The coefficients of variation were less than 2.67%. Results showed that the method was simple, rapid and accurate for TFs to be determined in black tea and TFs products.

导出引用

夏文娟, 张丽霞, 史作安, 贾明. 毛细管电泳分析茶黄素的方法研究[J]. 茶叶科学. 2006, 26(2): 147-153 https://doi.org/10.13305/j.cnki.jts.2006.02.014

XIA Wen-juan, ZHANG Li-xia, SHI Zuo-an, JIA Ming. Analysis of Theaflavins by Capillary Electrophoresis[J]. Journal of Tea Science. 2006, 26(2): 147-153 https://doi.org/10.13305/j.cnki.jts.2006.02.014

中图分类号： S571.1 R972+.6

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