EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响

doi:10.13305/j.cnki.jts.2006.03.013

茶叶科学 ›› 2006, Vol. 26 ›› Issue (3): 219-224.doi: 10.13305/j.cnki.jts.2006.03.013

EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响

陈勋¹, 于海宁², 沈生荣¹

1. 浙江大学茶叶研究所, 浙江杭州 310029;
2. 浙江工业大学药学院,浙江杭州 310032

收稿日期:2006-01-19 修回日期:2006-05-27 出版日期:2006-09-25 发布日期:2019-09-11
作者简介:陈勋（1979-）,男,湖北武穴人,硕士研究生,主要从事茶叶生物化学与综合利用方面的研究。
基金资助:
国家自然科学基金（30470198）和博士后基金资助项目（2004036486）

Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells

CHEN Xun¹, YU Hai-ning², SHEN Sheng-rong¹

1. Institute of Tea Sciences, Zhejiang University, Hangzhou 310029, China;
2. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China

Received:2006-01-19 Revised:2006-05-27 Online:2006-09-25 Published:2019-09-11

摘要/Abstract

摘要： 本文采用MTT法研究了EGCG以及EGCG与锌离子相互作用对前列腺癌细胞PC-3生长的影响;采用原子吸收光谱法(AAS)研究了锌离子及EGCG共存对PC-3细胞锌、镉离子吸收的影响;利用HPLC测定了锌离子对EGCG在PBS缓冲液与正辛醇,水与正辛醇体系中分配系数的影响。结果表明,EGCG能抑制前列腺癌细胞生长,细胞存活率呈时间和剂量依赖性;锌离子能抑制PC-3细胞的生长,促进EGCG对PC-3细胞生长的抑制作用,呈时间和剂量依赖性;同时,锌离子能抑制PC-3细胞对镉离子的吸收,而EGCG有增强效应。

关键词: 儿茶素, Zn²⁺, Cd²⁺, PC-3细胞

Abstract: In the present paper, effects of EGCG and interactions of EGCG with Zn²⁺on the growth of androgen-insensitive human prostate cancer cells (PC-3) were investigated by MTT assay. Effects of EGCG and Zn²⁺ on absorption of zinc and cadmium in PC-3 cells were detected by Flame Atomic Absorption Spectrometry (AAS) assay; and also the changes of Zn²⁺ on the partition coefficients of EGCG in n-octanol/PBS or n-octanol / water system were determined by HPLC technique. EGCG was screened for growth inhibition of PC-3 cells in dose- and time-dependent manners. Both Zn²⁺ and EGCG decreased viability of PC-3 cells in dose- and time-dependent relations, and Zn²⁺ ameliorated the effect of EGCG on the growth of PC-3 cells. Also, Zn²⁺ decreased the absorption of PC-3 cells on Cd²⁺, and EGCG could enhance the effect as well.

Key words: catechins, Zn²⁺, Cd²⁺, PC-3 cells

中图分类号:

S571.1
R983

陈勋, 于海宁, 沈生荣. EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响[J]. 茶叶科学, 2006, 26(3): 219-224. doi: 10.13305/j.cnki.jts.2006.03.013.

CHEN Xun, YU Hai-ning, SHEN Sheng-rong. Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells[J]. Journal of Tea Science, 2006, 26(3): 219-224. doi: 10.13305/j.cnki.jts.2006.03.013.

参考文献 21

[1]	Rebbeck T R.Genetics, disparities, and prostate cancer[J]. LDI Issue Brief 2005, 10(7): 1~4.
[2]	Landis S H, Murray T, Bolden S, et al. Cancer statistics[J]. Cancer J Clin, 1998, 48(6~29): 43~62.
[3]	Nakazato T, Ito K, Miyakawa Y, et al. Catechin, a green tea component, rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo[J]. Haematologica, 2005, 90(3): 317~325.
[4]	Kinlen, L J, Willows A N, Goldblatt P, et al. Tea consumption and cancer[J]. Brit J Cancer, 1988(58): 397~401.
[5]	Heilburn L K, Nomura A, Stemmermann, G N.Black teaconsumption and cancer risk: A prospective study[J]. Br J Cancer, 1986(54): 677~683.
[6]	Wynder E L, Rose D P, Cohen L A.Nutrition and prostate cancer: a proposal for dietary intervention[J]. Nutr Cancer, 1994(22): 1~10.
[7]	Hussain T, Gupta S, Adhami V M, et al. Green tea constituent epigallocatechin-3-gallate selectively inhibits COX-2 without affecting COX-1 expression in human prostate carcinoma cells[J]. Int J Cancer, 2005, 113(4): 660~669.
[8]	Hiipakka R A., Zhang H Z, Dai W, et al. Structure-activity relationships for inhibition of human 5alpha-reductases by polyphenols[J]. Biochem Pharmacol, 2002, 63(6): 1165~1176.
[9]	Brusselmans K, De Schrijver E, Heyns W, et al. Epigallocatechin-3-gallate is a potent natural inhibitor of fatty acid synthase in intact cells and selectively induces apoptosis in prostate cancer cells[J]. Int J Cancer, 2003, 106(6): 856~862.
[10]	Pezzato E., Sartor L, Dell'Aica I, et al. Prostate carcinoma and green tea: PSA-triggered basement membrane degradation and MMP-2 activation are inhibited by (-) epigallocatechin-3-gallate[J]. Int J Cancer, 2004, 112(5): 787-792.
[11]	Vayalil P K, Katiyar S K.Treatment of epigallocatechin-3-gallate inhibits matrix metalloproteinases-2 and -9 via inhibition of activation of mitogen-activated protein kinases, c-jun and NF-kappaB in human prostate carcinoma DU-145 cells[J]. Prostate 2004, 59(1): 33~42.
[12]	Ren F, Zhang S, Mitchell, S H, et al. Tea polyphenols down-regulate the expression of the androgen receptor in LNCaP prostate cancer cells[J]. Oncogene, 2000, 19(15): 1924~32.
[13]	Ishii,K., Otsuka T., Iguchi K., et al. Evidence that the prostate-specific antigen (PSA)/Zn2+ axis may play a role in human prostate cancer cell invasion[J]. Cancer Lett,2004, 207(1):79~87.
[14]	Scudiero D A, Shoemarker R H, Paull K D, et al. Evaluation of a soluble tetrazolium/formaza assay for cell growth and drug sensitivity in culture using human and other tumor cell lines[J]. Cancer Res, 1998(48): 4827.
[15]	Ayhan D, Erol P, Fethiye G, et al. Adsorption of Cu(Ⅱ), Zn, 2005(282): 20~25.
[16]	Liang Y R.Analysis of theaflavins by HPLC-photodiode arraydetector. Acta[J]. Agriculture.Universitati 1992, 18(s): 70~74.
[17]	Tang D S, Shen S R, Chen X., et al. Interaction of catechins with aluminum in vitro[J]. J Zhejiang Univ Sci 2004, 5(6): 668~675.
[18]	K Midori, S Tamiyoshi, Kinuyo, et al. Effects of pH and metal ions on antioxidative activities of catechins[J]. Biosci Biotechnol Biochem, 2000(65): 126~132.
[19]	Ogumlewa J O, Osegbe D N.Zinc and cadmium concentrations in indigenous blacks with normal, hypertrophic and malignante prostate[J]. Cancer, 1989(63): 1388~1392.
[20]	Satoh M, Koyama H, Kaji T, et al. Perspectives on cadmium toxicity research[J]. Tohoku J Exp Med 2002, 196(1): 23~32.
[21]	Reed M J.The uptake of testosterone and Zinc in vitro by the human hypertrophic prostate[J]. J Endo, 1973(58): 405.

EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响

Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells

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EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响

Effect of Interactions Between EGCG and Zn2+ on the Growth of Prostate Cancer PC-3 Cells

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Effect of Interactions Between EGCG and Zn²⁺ on the Growth of Prostate Cancer PC-3 Cells