基于DDRT-PCR研究茶树对茶尺蠖取食诱导的基因表达谱差异

doi:10.13305/j.cnki.jts.2007.02.007

茶叶科学 ›› 2007, Vol. 27 ›› Issue (2): 133-140.doi: 10.13305/j.cnki.jts.2007.02.007

基于DDRT-PCR研究茶树对茶尺蠖取食诱导的基因表达谱差异

韦朝领^{1, 2, *}, 高香凤¹, 叶爱华¹, 杨云秋¹, 江昌俊¹

1. 安徽农业大学农业部茶叶生物化学与生物技术重点开放实验室;
2. 安徽农业大学资源与环境科学学院, 安徽合肥230036

收稿日期:2007-02-08 修回日期:2007-03-08 出版日期:2007-06-25 发布日期:2019-09-11
作者简介:韦朝领（1968— ）,男,安徽舒城人,博士,硕士生导师,副教授,主要研究茶树生理与生物技术等。weichl@ahau.edu.cn
基金资助:
安徽省自然科学基金（批准号050410103）和国家自然科学基金（批准号：30671331）资助

Differential Gene Expression Profiles Analysis of Tea Plant Induced by Tea Looper (Ectropic oblique) Attack Using DDRT-PCR

WEI Chao-ling^{1, 2, *}, GAO Xiang-feng¹, YE Ai-hua¹, YANG Yun-qiu¹, JIANG Chang-jun¹

1. Key Laboratory of Tea Bio-chemistry and technology,Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China;
2. School of Resources and Environmental Science, Anhui Agricultural University, Hefei 230036, China

Received:2007-02-08 Revised:2007-03-08 Online:2007-06-25 Published:2019-09-11

摘要/Abstract

摘要： 利用DDRT-PCR技术初步研究了茶树被害虫茶尺蠖取食后基因表达谱差异。结果表明：接头引物A3、A4、A6、A7和A8分别与随机引物R1-R8,R12组合扩增后,总共得到222条差异片段,占总条带数的32.8％。在所鉴定的20个差异片段中,有8个片段序列没有发现同源序列;有5个片段序列与未知功能蛋白相关;其余7个差异表达的片段序列可分为6类,即分别与光合系统II色素蛋白（C15）、非生物抗性诱导蛋白（D22）、糖苷代谢酶（A45）、核酸和蛋白转录调控因子（A12, D63）、生长素调节蛋白（E94）和营养性抗虫蛋白（D73）。其中差异表达片段C15、A45、D22、D73和E94在植物与害虫相互作用相关分子机制研究中首次被发现。

关键词: 茶树, 茶尺蠖, 取食诱导, 基因表达谱差异显示

Abstract: DDRT-PCR technique was used to explore the differential gene expression profiles analysis of tea plant induced by tea looper (Ectropic oblique) attack. Results showed that 222 differential expression fragments were obtained, which was 32.8% of total fragments, by amplified in anchor primers A3, A4, A6, A7, A8 and random primers R1-R8,R12, respectively. Twenty fragments of which were re-amplified by RT-PCR. Based on the BLASTx search in GenBank, it were found that eight of the fragments shared no homology, five of the fragments shared homology with the unknown proteins, and seven of the fragments shared higher score homology with the known proteins, which can be divided into six groups related to photosynthetic pigment protein in photosystem II(C15), abiotic resistance induced protein(D22), glucoside metabolism enzyme(A45), nucleic acid and protein transcription and regulation factors(A12, D63), auxin-regulated protein(E94) and VIP2 protein(D73). The fragments of C15, A45, D22, D73 and E94 were firstly found in the studies of molecular mechanism of plant-insect interaction, up to day.

Key words: tea plant, Ectropic oblique, damage induction, differential display for gene expression profile

中图分类号:

S571.1
Q523

韦朝领, 高香凤, 叶爱华, 杨云秋, 江昌俊. 基于DDRT-PCR研究茶树对茶尺蠖取食诱导的基因表达谱差异[J]. 茶叶科学, 2007, 27(2): 133-140. doi: 10.13305/j.cnki.jts.2007.02.007.

WEI Chao-ling, GAO Xiang-feng, YE Ai-hua, YANG Yun-qiu, JIANG Chang-jun. Differential Gene Expression Profiles Analysis of Tea Plant Induced by Tea Looper (Ectropic oblique) Attack Using DDRT-PCR[J]. Journal of Tea Science, 2007, 27(2): 133-140. doi: 10.13305/j.cnki.jts.2007.02.007.

参考文献 18

[1]	Hermsmeier D, Schittko U, Baldwin I T.Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural host nicotiana attenuata. Ⅰ. large-scale changes in the accumulation of growth- and defense-related plant mRNAs[J]. Plant Physiology, 2001, 125:683~700.
[2]	韦朝领, 高香凤. 基因表达谱差异显示技术及其在植物对害虫取食诱导反应研究中的应用[J]. 安徽农业大学学报, 2006, 33(1): 94~99.
[3]	Voelcke C, Baldwin I T.Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata: V. Microarray Analysis and Further Characterization of Large-Scale Changes in Herbivore-Induced mRNAs[J]. Plant Physiol, 2003, 131: 1877~1893.
[4]	张汉鹄. 我国茶树尺蛾区系考察[J]. 茶叶科学, 2001, 21(2): 157~160.
[5]	许宁, 陈宗懋, 游小清. 引诱茶尺蠖天敌寄生蜂的茶树挥发物的分离与鉴定[J]. 昆虫学报, 1999, 42(2): 129~130.
[6]	赵冬春, 陈宗懋, 程家安. 茶树-假眼小绿叶蝉-白斑猎蛛间化学通讯物的分离与活性鉴定[J]. 茶叶科学, 2002, 22(2): 109~114.
[7]	Hui DQ, Iqba J, Lehmann K, et al. Molecular interactions between the specialist herbivore Manduca Sexta (Lepoidoptera, Sphingidae) and the nature host Nicotiana attenuata:V.Microarray analysis and further characterization oflarge-scale changes in herbivore-induced mRNAs[J]. Plant Physiol, 2003, 131: 1877~1893.
[8]	Hermsmeier D, Mazarei M, Baum TJ.Differential display analysis of the early compatible interaction between soybean and the soybean cyst nematode[J]. Molecular Plant-Microbe Interactions, 1998, 11(12): 1258~1263.
[9]	Bauer D, Muller H, Reich J, et al. Identification of differentially expressed mRNA species by an improved display technique(DDRT-PCR)[J]. Nucleic Acids Research, 1993, 21(18): 4272~4280.
[10]	pursiheimo S, martinsuo P, rintamäki E, et al. Photosystem II protein phosphorylation follows four distinctly different regulatory patterns induced by environmental cues[J]. Plant, Cell and Environment, 2003(26): 1995~2003.
[11]	Retuerto R, Fernandez-lema B, Rodriguez-roiloa et al. Increased photosynthetic performance in holly trees infested by scale insects[J]. Functional Ecology, 2004(18): 664~669.
[12]	Zheng-Zhu Zhang, Ying-Bo Li, Li Qi, et al. Antifungal Activities of Major Tea Leaf Volatile Constituents toward Colletorichum camelliae Massea[J]. J Agric Food Chem, 2006, 54(11): 3936~3940.
[13]	陈宗懋, 许宁, 韩宝瑜, 等. 茶树-害虫-天敌间的化学信息联系[J]. 茶叶科学, 2003, 23(增): 38~45.
[14]	许宁, 陈宗懋, 游小清. 三级营养关系中茶树间接防御茶尺蠖危害的生化机制[J]. 茶叶科学, 1998, l8(1): 1~5.
[15]	Martínez G A, Chaves A R, Civello P M.β-xylosidase activity and expression of a b-xylosidase gene during strawberry fruit ripening[J]. Plant Physiology and Biochemistry, 2004(42): 89~96.
[16]	Ruperti B, Cattivelli L, Pagni S, et al. Ethylene-responsive genes are differentially regulated during abscission, organ senescence and wounding in peach(Prunus persica)[J]. J Exp Bot, 2002(53): 429~437.
[17]	Sung DY.Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family[J]. Plant Physiol, 2001, 126: 789~800.
[18]	Reddy A.R., Ramakrishna W, Sekhar A C, et al. Novel genes are enriched in normalized cDNA libraries from drought-stressed seedlings of rice(Oryza sativa L. subsp. indica cv. Nagina 22)[J]. Genome, 2002(45): 204~211.

基于DDRT-PCR研究茶树对茶尺蠖取食诱导的基因表达谱差异

Differential Gene Expression Profiles Analysis of Tea Plant Induced by Tea Looper (Ectropic oblique) Attack Using DDRT-PCR

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 18

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	王留彬, 黄丽蕴, 滕翠琴, 吴立赟, 成浩, 于翠平, 王丽鸳. 梧州茶树种质资源的遗传多样性及亲缘关系分析[J]. 茶叶科学, 2022, 42(5): 601-609.
[2]	周汉琛, 杨霁虹, 徐玉婕, 吴琼, 雷攀登. 香叶醇生物合成相关基因NUDX1的进化分析[J]. 茶叶科学, 2022, 42(5): 638-648.
[3]	陈琪予, 马建强, 陈杰丹, 陈亮. 利用图像特征分析茶树成熟叶表型的遗传多样性[J]. 茶叶科学, 2022, 42(5): 649-660.
[4]	孙悦, 吴俊, 韦朝领, 刘梦月, 高晨曦, 张灵枝, 曹士先, 余顺甜, 金珊, 孙威江. 抗小贯松村叶蝉和茶棍蓟马的茶树种质筛选及其抗性相关因素分析[J]. 茶叶科学, 2022, 42(5): 689-704.
[5]	王玉源, 刘任坚, 刘少群, 舒灿伟, 孙彬妹, 郑鹏. 茶树R2R3-MYB转录因子CsTT2表达分析及功能初步鉴定[J]. 茶叶科学, 2022, 42(4): 463-476.
[6]	刘建军, 张金玉, 彭叶, 刘晓博, 杨云, 黄涛, 温贝贝, 李美凤. 不同光质摊青对夏秋茶树鲜叶挥发性物质及其绿茶品质影响研究[J]. 茶叶科学, 2022, 42(4): 500-514.
[7]	汪为通, 周孝贵, 张欣欣, 王志博, 张大羽, 肖强. 条纹蝇虎对灰茶尺蠖幼虫的捕食作用[J]. 茶叶科学, 2022, 42(4): 515-524.
[8]	邢安琪, 武子辰, 徐晓寒, 孙怡, 王艮梅, 王玉花. 茶树富集氟的特点及其机制的研究进展[J]. 茶叶科学, 2022, 42(3): 301-315.
[9]	王涛, 王艺清, 漆思雨, 周喆, 陈志丹, 孙威江. 茶树CLH基因家族的鉴定与转录调控研究及其在白化茶树中的表达分析[J]. 茶叶科学, 2022, 42(3): 331-346.
[10]	刘任坚, 王玉源, 刘少群, 舒灿伟, 孙彬妹, 郑鹏. 茶树CsbHLH024和CsbHLH133转录因子功能鉴定[J]. 茶叶科学, 2022, 42(3): 347-357.
[11]	欧阳珂, 张成, 廖雪利, 坤吉瑞, 童华荣. 基于感官组学分析玉米香型南川大茶树工夫红茶特征香气[J]. 茶叶科学, 2022, 42(3): 397-408.
[12]	刘富浩, 范延艮, 王域, 孟凡月, 张丽霞. 茶树黄金芽CsHIPP26.1蛋白螯合离子的筛选与鉴定[J]. 茶叶科学, 2022, 42(2): 179-186.
[13]	杨妮, 李逸民, 李静文, 滕瑞敏, 陈益, 王雅慧, 庄静. 外源5-ALA对干旱胁迫下茶树叶绿素合成和荧光特性及关键酶基因表达的影响[J]. 茶叶科学, 2022, 42(2): 187-199.
[14]	严玉婷, 李玉杰, 王倩, 唐美君, 郭华伟, 李红亮, 孙亮. 化学感受蛋白直系同源基因CSP8在茶尺蠖及近缘种灰茶尺蠖中的表达分析[J]. 茶叶科学, 2022, 42(2): 200-210.
[15]	疏再发, 郑生宏, 邵静娜, 周慧娟, 吉庆勇, 刘瑜, 何卫中, 王丽鸳. 不同茶树品种（系）对减半施肥的响应研究[J]. 茶叶科学, 2022, 42(2): 277-289.