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茶叶科学 ›› 2007, Vol. 27 ›› Issue (2): 159-162.doi: 10.13305/j.cnki.jts.2007.02.011

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茶叶中亚硝酸盐检测技术研究

古小玲, 鲁成银*, 刘新, 于良子, 陈利燕, 汪庆华   

  1. 中国农业科学院茶叶研究所/农业部茶叶质量监督检验测试中心,浙江 杭州 330008
  • 收稿日期:2007-02-08 修回日期:2007-03-06 出版日期:2007-06-25 发布日期:2019-09-11
  • 通讯作者: *鲁成银,lchy@mail.tricaas.com
  • 作者简介:古小玲(1980— ),女,江西遂川人,硕士研究生,从事茶叶亚硝酸盐检测标准研究。
  • 基金资助:
    浙江省重点科研社会发展项目(2006C23089)研究内容之一

The Detection Method of Nitrite in Tea

GU Xiao-ling, LU Cheng-yin*, LIU xin, YU Liang-zi, CHEN-Li-yan, WANG-Qing-hua   

  1. Tea Quality Supervision and Inspection Centre Ministry of Agriculture; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2007-02-08 Revised:2007-03-06 Online:2007-06-25 Published:2019-09-11

摘要: 利用在酸性条件下亚硝酸盐与对氨基苯磺酸重氮化后,再与盐酸萘乙二胺偶合形成颜色反应,建立了用比色法测定茶叶中亚硝酸盐的方法。针对茶叶中存在的茶多酚、茶色素和抗坏血酸等干扰因素,优化了测定的最佳技术参数。建立的方法在所测的范围内具有良好的线性(相关系数为0.9993),变异系数为4.6%,回收率为72.90%~86.53%。本方法快速,准确、操作简单,适用于茶叶中的亚硝酸盐含量的测定。

关键词: 茶叶, 亚硝酸盐, 检测

Abstract: A spectophotometric method on the determination of nitrite in tea was investigated, which is based on the diazotization of nitrite with p-aminobenzene sulfonic acid under acidic conditions then coupled with N-(1-Naphthyl)- ethylenediamine dihydrochloride in forming the colour-producing response. Because of the interference of polyphenols, pigment and ascorbic acid in tea, the optimum operating conditions of measurement was established. The method showed a good analytical characteristics with correlation coefficients of 0.9993, variation coefficient of 4.6%, and recovery was ranging from 72.90% to 86.53%. It is a rapid, accurate, simple method and applicable for the determination of nitrite in tea.

Key words: tea, nitrite, detection

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