膜过滤绿茶多糖的系统分级纯化及免疫活性分析

寇小红; 江和源; 崔宏春; 张建勇; 高晴晴; 袁新跃; 舒爱民; 刘晓辉; 高琪

doi:10.13305/j.cnki.jts.2008.03.011

PDF(341 KB)

茶叶科学 ›› 2008, Vol. 28 ›› Issue (3) : 172-180. DOI: 10.13305/j.cnki.jts.2008.03.011

膜过滤绿茶多糖的系统分级纯化及免疫活性分析

寇小红^1,2, 江和源^1,*, 崔宏春^1,2, 张建勇¹, 高晴晴^1,2, 袁新跃¹, 舒爱民¹, 刘晓辉^1,2, 高琪¹

作者信息 +

Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities

KOU Xiao-hong^1,2, JIANG He-yuan^1,*, CUI Hong-chun^1,2, ZHANG Jian-yong¹, GAO Qing-qing^1,2, YUAN Xin-yue¹, SHU Ai-min¹, LIU Xiao-hui^1,2, GAO Qi¹

Author information +

文章历史 +

摘要

采用150 kD、20 kD、6 kD的超滤膜和对水溶性茶多糖进行分级和浓缩,得到的三部分截留液分别经过DEAE-52纤维素离子交换层析和丙烯葡聚糖凝胶Sephacryl S-300柱层析系统分离、纯化,共得到了20多个分级组分;HPGPC-ELSD鉴定各多糖组分的纯度及分子量分布,结果表明得到5个均一多糖组分;用小鼠巨噬细胞系Raw264.7检测免疫活性,发现对小鼠巨噬细胞Raw264.7有显著活性的多糖组分大多集中在20 kD左右,大多为不均一多糖。

Abstract

Water-soluble green tea polysaccharides were separated and purified by Ultrafiltration membranes with pore diameter of 150 kD, 20 kD, 6 kD in turn. All the three fractions were further separated and purified by DEAE-cellulose ion-exchange chromatography and Sephacryl S-300 column chromatography, and more than 20 green tea polysaccharide components totally were obtained. Their purity and molecular weight distribution were determined with high performance gel permeation chromatography (HPGPC) equipped with ELSD detector, and five components of homogeneous polysaccharide were obtained. The immunological activities of these green tea polysaccharides were determined by stimulated phagocytosis and nitric oxide production on RAW264.7 macrophage cell line of mice. Results showed that the tea Polysaccharides with 20 kD MW possessed significant immunological activities on NO production or phagocytosis of mice macrophage Raw 264.7 cells.

导出引用

寇小红, 江和源, 崔宏春, 张建勇, 高晴晴, 袁新跃, 舒爱民, 刘晓辉, 高琪. 膜过滤绿茶多糖的系统分级纯化及免疫活性分析[J]. 茶叶科学. 2008, 28(3): 172-180 https://doi.org/10.13305/j.cnki.jts.2008.03.011

KOU Xiao-hong, JIANG He-yuan, CUI Hong-chun, ZHANG Jian-yong, GAO Qing-qing, YUAN Xin-yue, SHU Ai-min, LIU Xiao-hui, GAO Qi. Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities[J]. Journal of Tea Science. 2008, 28(3): 172-180 https://doi.org/10.13305/j.cnki.jts.2008.03.011

中图分类号： TS272 Q539

参考文献

[1] 傅博强, 谢明勇, 周鹏. 茶叶多糖的提取纯化、组成及药理作用研究进展[J]. 南昌大学学报(理科版), 2001, 25(4): 358~364.
[2] 陈海霞, 谢笔钧. 茶多糖的不同提取工艺的比较研究[J]. 食品工业科技, 2001, 22(2): 18.
[3] Haixia Chen, Min Zhang, Bijun Xie.Components and antioxidant activity of polysaccharide conjugate from green tea[J]. Food Chemistry, 2005(90): 17~21.
[4] 王元凤. 茶多糖分离纯化结构及构效关系研究[D]. 无锡: 江南大学, 2005.
[5] 施兆鹏. 茶叶加工学[M]. 北京: 中国农业出版社, 1997: 6~8.
[6] 王立生, 潘令嘉, 施理, 等. 双歧杆菌的全肽聚糖对裸鼠腹腔巨噬细胞产生IL-6、IL-12及一氧化氮的影响[J]. 中国病理生理杂志, 2000, 16(2): 153~156.
[7] 陈春锋, 杨晓彤, 李绪全, 等. 5个不同灵芝种菌丝体多糖理化性质及免疫活性研究[J]. 微生物学报, 2007, 47(4): 68~73.
[8] Cao LZ, Lin ZB.Comparision of the effects of polysaccharides from wood-cultured and bag-cultured Ganoderma Lucidum on murine spleen lymphocyte proliferation in vivo[J]. Acta pharmaccutica Sinica, 2003, 38(2): 92~97.
[9] Cao LZ, Lin ZB.Antitumor and anti-angiogenic activity of Ganoderma Lucidum polysaccharides peptide[J]. Acta pharma Sin, 2004, 25(6): 833~838.
[10] Fang QH, Zhang JJ.Submerged fermentation of higher fungus Ganoderma Lucidum for production of valuable bioactive metabolites-ganoderic acid and polysaccharide[J]. Biochem Eng J, 2002, 10: 61~65.
[11] Chang MY, Tsai GJ, Hong JY.Optimization of the medium composition for the submerged culture of Ganoderma Lucidum by Taguchi array design and steepest ascent method[J]. Enzyme Microb Technol, 2006, 38: 407~414.
[12] 李布青, 张慧玲. 中低档绿茶中茶多糖的提取及降血糖作用[J]. 茶叶科学, 1996, 16(1): 67~72.
[13] 许新德, 高荫榆, 陈才水, 等. 茶叶多糖的纯化及组分研究[J]. 食品科学, 2000, 21(8): 13~15.
[14] 任健, 刘钟栋, 陈肇锬. 信阳毛尖种茶多糖的提取、纯化与组分研究[J]. 郑州工程学院学报, 2003, 24(2): 1~4.
[15] 周鹏, 谢明勇, 傅博强, 等. 茶叶粗多糖的提取及纯化研究[J]. 食品科学, 2001, 22(11): 46~47.
[16] 汪东风, 谢晓风. 茶多糖的组分及理化性质[J]. 茶叶科学, l996, 16(1): l~8.
[17] 王丁刚, 王淑如. 茶叶多糖的分离、纯化、分析及降血脂作用[J]. 中国药科大学学报, 1991, 22(4): 225~228.
[18] 孙玉军, 陈彦. 茶叶多糖的分离纯化及其结构分析概述[J]. 中国茶叶加工, 2004(4): 38~40.