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茶叶科学 ›› 2009, Vol. 29 ›› Issue (1): 15-19.doi: 10.13305/j.cnki.jts.2009.1.003

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奶茶中三聚氰胺的HPLC测定方法

江和源1, 袁新跃1,2, 张建勇1, 崔宏春1,2, 刘晓辉1,2, 尧渝1,2   

  1. 1. 中国农业科学院茶叶研究所,农业部茶及饮料作物产品加工与质量控制重点实验室,浙江 杭州 310008;
    2. 中国农业科学院研究生院,北京 100081
  • 收稿日期:2008-10-28 修回日期:2008-11-27 出版日期:2009-02-15 发布日期:2019-09-06
  • 作者简介:江和源(1974— ),男,江西都昌人,博士,副研,主要从事天然产物化学、茶叶化学与加工研究。jianghy@mail.tricaas.com
  • 基金资助:
    国家自然科学基金(30570191)和浙江省钱江人才计划(QJD0702012)资助

Determination of Melamine in Milk Tea By High Performance Liquid Chromatography

JIANG He-yuan1, YUAN Xin-yue1,2, ZHANG Jian-yong1, CUI Hong-chun1,2, LIU Xiao-hui1,2, YAO Yu1,2   

  1. 1. Key Laboratory of Tea and Beverage Crops Processing and Quality Control, Tea Research Institute, Chinese Academy of Agriculture Sciences, Hangzhou 310008, China;
    2. Graduate School, Chinese Academy of Agriculture Sciences, Beijing 100081, China
  • Received:2008-10-28 Revised:2008-11-27 Online:2009-02-15 Published:2019-09-06

摘要: 建立了奶茶中三聚氰胺的HPLC测定方法。适用检测奶茶中三聚氰胺的HPLC条件为:色谱柱Sunfire C18柱(250 mm×4.6 mm ID: 5 μm),流动相由0.008 mol/L辛烷磺酸钠和乙腈组成,柱温30℃,流速1.0 ml/min,检测波长236 nm,外标法定量。分析了市场上奶茶产品的三聚氰胺含量,发现少数奶茶产品中三聚氰胺的含量超过了国家暂行限量标准的2.5 mg/kg。

关键词: 高效液相色谱, 奶茶, 三聚氰胺

Abstract: The method to determinate melamine in milk tea by HPLC, was established. The samples were analyzed by a Sunfire C18 column (250 mm×4.6 mm ID; 5 μm). The mobile phase was composed of 0.008 mol/L sodium 1-Octanesulfonate (adjust to pH3.3 with acetic acid) and acetonitrile. The flow rate was 1.0 ml/min. Column temperature was 30℃. UV-detection was performed at 236 nm. The contents were quantified with exterior standard. Some milk tea samples from the supermarket were analyzed, and results showed that the content of melamine in some milk tea were higher than 2.5 mg/kg, the set limit in national standard.

Key words: HPLC, milk tea, melamine

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