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茶叶科学 ›› 2009, Vol. 29 ›› Issue (5): 379-384.doi: 10.13305/j.cnki.jts.2009.5.008

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茶叶中EGCG3″Me的分析方法研究

孙业良1,2, 吕海鹏2, 林智2,*, 黎星辉1,*   

  1. 1. 南京农业大学茶叶研究所,江苏 南京 210095;
    2. 中国农业科学院茶叶研究所/茶叶加工工程研究中心,浙江 杭州 310008
  • 收稿日期:2009-03-23 修回日期:2009-04-10 出版日期:2009-10-15 发布日期:2019-09-09
  • 通讯作者: *
  • 作者简介:孙业良(1983— ),男,山东潍坊人,硕士研究生,研究方向为茶叶化学和茶叶加工。
  • 基金资助:
    国家科技支撑计划(2006BAD06B01); 浙江省自然科学基金项目(Y307498和Y3080088)

Study on the Analytical Method of EGCG 3″Me by HPLC in Tea

SUN Ye-liang1,2, LV Hai-peng2, LIN Zhi2,*, LI Xing-hui1,*   

  1. 1.Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2. Engineering Research Center for Tea Processing; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2009-03-23 Revised:2009-04-10 Online:2009-10-15 Published:2019-09-09

摘要: 采用制备HPLC系统进行分离纯化并进一步结合NMR、LC/MS进行结构鉴定,从茶叶中分离纯化出了EGCG3″Me;研究了不同提取条件对茶叶中EGCG3″Me含量的影响,初步建立了用反相高效液相色谱测定茶叶中EGCG3″Me含量的分析方法。结果表明,该方法重现性好,适用于常规定量分析,平均加样回收率为95.48%,RSD为3.31%;采用蒸馏水在90℃的水浴浸提5 min,茶汤中EGCG3"Me的浓度可以达到最高值。

关键词: 茶, EGCG3"Me, 分析, 含量

Abstract: EGCG3″Me was isolated by preparative HPLC system from tea and identified by NMR and LC/MS. The influence of different extracting method on the EGCG3″Me content in tea was discussed, and a simple high performance liquid chromatography analytical method of EGCG3″Me was developed. Results showed that the analytical method showed a good linear relationship between the peak area and content of EGCG3″Me (R2=0.9998) and high reproducibility. The average recovery rate of EGCG3″Me was 95.48% and the relative standard deviation(RSD)was 3.31%. The EGCG3″Me concentration in tea infusion can reach highest when the tea sample was immersed in distilled water for 5 min at 90℃.

Key words: tea, EGCG3"Me, analysis, content

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