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茶叶科学 ›› 2010, Vol. 30 ›› Issue (5): 362-366.doi: 10.13305/j.cnki.jts.2010.05.006

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低磷胁迫下茶树根系CS基因的克隆及表达分析

林郑和, 陈常颂, 邬龄盛   

  1. 福建省农业科学院茶叶研究所,福建 福安 355000
  • 收稿日期:2010-04-22 修回日期:2010-06-02 发布日期:2019-09-11
  • 作者简介:林郑和(1975— ),男,福建福安人,博士,主要从事茶树生理生化与分子生物学方面的研究。
  • 基金资助:
    福建省自然科学基金(2009J01083),科技部支撑计划(2007BAD07B02),国家茶叶产业技术体系(nycytx-23),福建省农科院创新团队“特色园艺作物育种与技术创新”

Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress

LIN Zheng-he, CHEN Chang-song, WU Ling-sheng   

  1. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu’an 355000, China
  • Received:2010-04-22 Revised:2010-06-02 Published:2019-09-11

摘要: 柠檬酸合成酶(Citrate Synthase, CS)是茶树体内合成柠檬酸的关键性酶。克隆了茶树根系柠檬酸合成酶基因片段,GenBank登录号为FJ814766,且利用半定量RT-PCR方法,研究该基因在低磷下的表达变化。结果表明,当供磷浓度为0(缺磷)时,根系CS基因的表达略有增强,这可能是茶树对低磷的一种适应机制。

关键词: 半定量RT-PCR, 低磷胁迫, 基因表达, 克隆, 茶树

Abstract: Citrate synthase(CS) is the key enzyme of producing citrate acid. CS gene fragments were cloned from tea roots. The GenBank accession is FJ814766 in the NCBI. Then the changes in gene expression in response to P supply were investigated by using semi-quantitative RT-PCR. The result showed that P deficiency increased the expressions of CS gene. This may be a tolerant mechanism of tea seedlings to P deficiency.

Key words: semi-quantitative RT-PCR, low P stress, gene expression, cloning, tea plant

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