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茶叶科学 ›› 2011, Vol. 31 ›› Issue (4): 295-299.doi: 10.13305/j.cnki.jts.2011.04.010

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对蓟马类害虫高致病性球孢白僵菌的分离、鉴定

王俊平, 郑长英*   

  1. 青岛农业大学农学与植物保护学院,山东 青岛 266109
  • 收稿日期:2011-03-03 修回日期:2011-03-28 出版日期:2011-08-15 发布日期:2019-09-09
  • 通讯作者: zhengcy67@qau.edu.cn
  • 作者简介:王俊平(1976— ),女,博士,主要从事害虫生物防治方面的研究。
  • 基金资助:
    国家公益性行业(农业)科研专项经费项目(200803025)、青岛农业大学高层次人才科研基金(630609)

Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips

WANG Jun-ping, ZHENG Chang-ying*   

  1. College of Agronomy and Plant Protection, Qingdao Agricultural university, Qingdao 266109, China
  • Received:2011-03-03 Revised:2011-03-28 Online:2011-08-15 Published:2019-09-09

摘要: 从草履蚧僵虫体内分离出12株病原真菌,对蓟马类昆虫具有70%~90%的致死率。现选出一株病原真菌CYT10利用形态和分子的特点进行鉴定。扫描电镜下观察,该菌产孢细胞簇生瓶状,“之”型产孢轴。经PCR获得rDNA-ITS和EF1-a核苷酸序列,与其他球孢白僵菌的相应序列一致性达95%以上,进化分析CYT10菌株与球孢白僵菌Beauveria bassiana聚为一类。根据菌株的形态观察和DNA序列分析确定该菌为球孢白僵菌。

关键词: 球孢白僵菌, 蓟马, 形态特点, rDNA-ITS, EF1-a

Abstract: 12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality. CYT10 strain was identified based on the morphological and molecular characteristics. Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced. The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana (>95%). Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain, which was belonging to B. bassiana. CYT10 strain was classified as a member of the species B. bassiana based on the morphological characteristics and analysis of DNA sequences.

Key words: Beauveria bassiana, thrip, morphological characteristics, rDNA-ITS, EF1-a

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