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茶叶科学 ›› 2013, Vol. 33 ›› Issue (5): 436-442.doi: 10.13305/j.cnki.jts.2013.05.003

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近平滑假丝酵母(Candida parapsilosis)发酵普洱茶初探

李亚莉1, 秘鸣2, 魏珍珍1,3, 周红杰1,*   

  1. 1. 云南农业大学普洱茶学院,云南 昆明 650201;
    2. 昆明理工大学国土资源工程学院,云南 昆明 650093;
    3. 云南中茶茶业有限公司,云南 昆明 650200
  • 收稿日期:2013-01-06 修回日期:2013-02-07 出版日期:2013-10-30 发布日期:2019-09-04
  • 通讯作者: *
  • 作者简介:李亚莉(1976— ),女,云南临沧人,博士,硕士生导师,主要从事普洱茶加工和综合利用的研究。
  • 基金资助:
    国家自然科学基金项目(31260197)、云南省自然科学重点基金项目(2009CC005)、国家科技支撑计划项目课题(2007BAD58B02)、云南省科技厅富民强县计划项目(2011EB117)

Application of Candida parapsilosis GPT-5-11 for Pu-erh Tea Fermentation

LI Ya-li1, BI Ming2, WEI Zhen-zhen1,3, ZHOU Hong-jie1,*   

  1. 1. Pu-erh Tea College, Yunnan Agricultural University, Kunming 650201, China;
    2. Faculty of Land Resource Engineering, Kunming University of Science and Technology, Kunming 650093, China;
    3 Yunnan ChinaTea Co. Ltd., Kunming 650200, China
  • Received:2013-01-06 Revised:2013-02-07 Online:2013-10-30 Published:2019-09-04

摘要: 以晒青茶为原料,接种专利菌株近平滑假丝酵母(Candida parapsilosis)GPT-5-11(专利号:201010182948.5)发酵普洱茶。结果表明,接种菌株的普洱茶样品GABA含量为0.3052~1.5332βmg/g,显著高于对照0.0628~0.1047βmg/g,其中灭菌每翻接种液态菌的样品GABA含量最高,为1.5332βmg/g,达到GABA茶的标准。同时,接种该菌株进行发酵后,发酵茶样中的主要功能成分含量均较自然发酵茶叶中的含量高,具有传统普洱茶的品质风味,且在香气和滋味方面有自身的特点,滋味醇和、厚滑,香气纯正略带乳香。

关键词: γ-氨基丁酸(GABA);, GPT-5-11菌株, 普洱茶, 发酵

Abstract: Sun-dried green tea was fermented by inoculation of Candida parapsilosis GPT-5-11. HPLC analysis showed that the contents of GABA in fermented tea samples inoculated GPT-5-11 yeast were 0.3052~1.5332βmg/g, and significantly higher than that in control fermented tea samples. The content of GABA in fermented sample No. 7 was 1.5332βmg/g, and it meets the Japanese standards of GABA tea. Chemical analysis and sensory evaluation showed that the content of chemical components of samples fermented with GPT-5-11 yeast were higher than those of the controls, and have special features in aroma and color as well as the characteristics of traditional pu-erh tea.

Key words: γ-aminobutyric acid(GABA), Candida parapsilosis GPT-5-11, Pu-erh tea, fermentation

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