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茶叶科学 ›› 2013, Vol. 33 ›› Issue (6): 491-499.doi: 10.13305/j.cnki.jts.2013.06.008

• •    下一篇

氮素水平对茶树新梢叶片代谢谱及其昼夜变化的影响

杨亦扬1,2, 马立锋2, 黎星辉3, R. George Ratcliffe4, Nicholas J. Kruger4, 阮建云2,*   

  1. 1. 江苏省农业科学院园艺研究所,江苏 南京 210014;
    2. 中国农业科学院茶叶研究所,浙江 杭州 310008;
    3. 南京农业大学茶叶研究所,江苏 南京 210095;
    4. Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
  • 收稿日期:2013-03-21 修回日期:2013-10-04 出版日期:2013-12-30 发布日期:2019-09-04
  • 通讯作者: *jruan@mail.tricaas.com
  • 作者简介:杨亦扬(1983— ),女,江苏阜宁人,博士,主要从事茶树生理与营养研究。
  • 基金资助:
    国家茶叶产业技术体系(CARS23)、江苏省农业科技自主创新基金项目[CX(13)5016]、国家自然科学基金(30771251)

Metabonomic Analysis on the Effects of different Nitrogen Application Rates and Light/Dark Change on Metabolism in Tea Leaves

YANG Yi-yang1,2, MA Li-feng2, LI Xing-hui3, R. G. Ratcliffe4, N. J. Kruger4, RUAN Jian-yun2, *   

  1. 1. Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
    3. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    4. Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
  • Received:2013-03-21 Revised:2013-10-04 Online:2013-12-30 Published:2019-09-04

摘要: 茶树叶片中含有丰富的代谢产物,氨基酸、茶多酚和咖啡碱是决定茶叶品质的重要成分。本文结合基于1H-核磁共振(1H-NMR)的代谢组学非靶标分析与通过高效液相色谱法进行的定量靶标分析两种方法,比较白天和夜晚所取不同施氮水平的茶树新梢第2叶的代谢产物差异,探讨了基于1H-NMR的代谢组学研究方法应用于茶叶品质成分形成机理研究的可行性。结果表明,白天和夜晚所取茶树新梢第2叶代谢组差异较大,可以显著分离,区分两个样本的主要组分为茶氨酸、葡萄糖和蔗糖,而不同施氮水平下茶树新梢第2叶代谢组没有显著差异。高效液相色谱定量分析的结果表明,施氮水平对主要游离氨基酸组分和儿茶素组分均有影响,且存在昼夜差别。

关键词: 茶树, 施氮水平, 昼夜, 茶多酚, 氨基酸

Abstract: Amino acids, polyphenols, caffeine and other metabolites such as sugars and aromatic substances are important determinants of tea quality. Different cultivation practices affect tea quality by altering the basic metabolite profiles. In this study, the metabolomic changes through nitrogen and samples collected in day and night were investigated in fresh tea shoots by 1H-NMR for non-targeted analysis coupled with a multivariate data analysis and by HPLC for targeted analysis to reveal the mechanism of formation, transformation and relationship of the major characteristic quality components in fresh tea leaves. Non-targeted analysis with 1H-NMR showed significant effect of samples collected in day and night but no difference among N application rates. However, targeted analysis with HPLC showed both significant variations in amino acids and catechins as affected by N rates and light/dark change. There were higher sensitivity of amino acids and sugars but lower sensitivity of tea polyphenols through the metabolomic analysis using 1H-NMR.

Key words: Camellia sinensis, nitrogen application rate, light/dark, polyphenols, amino acids

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