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茶叶科学 ›› 2014, Vol. 34 ›› Issue (2): 195-203.doi: 10.13305/j.cnki.jts.2014.02.016

• • 上一篇    

基于数字表达谱对茶树类黄酮合成、调控相关基因表达的研究

韦康, 王丽鸳, 成浩*, 张丽群, 冯素花   

  1. 中国农业科学院茶叶研究所,国家茶树改良中心,浙江 杭州 310008
  • 收稿日期:2013-07-01 修回日期:2013-08-12 出版日期:2014-04-15 发布日期:2019-09-03
  • 通讯作者: *chenghao@mail.tricaas.com
  • 作者简介:韦康(1981— ),男,浙江东阳人,副研究员,主要从事茶树分子生物学研究。
  • 基金资助:
    现代农业产业技术体系建设专项资金资助(No. nycytx-23)、中央级公益性科研院所基本科研业务费专项(2012ZL049)

Analysis of Differential Gene Expression on Flavonoid Biosynthesis and Its Regulation in Tea Plant (Camellia sinensis L.)

WEI Kang, WANG Liyuan, CHENG Hao*, ZHANG Liqun, FENG Suhua   

  1. Tea Research Institute, Chinese Academy of Agriculture Science, National Center for Tea Improvement, Hangzhou 310008, China
  • Received:2013-07-01 Revised:2013-08-12 Online:2014-04-15 Published:2019-09-03

摘要: 利用数字基因表达谱技术研究了茶树类黄酮合成及调控相关基因在花瓣、花粉、休眠芽、萌发芽中的表达情况。结果发现花瓣、休眠芽、萌发芽中类黄酮合成基因大量表达,而花粉中类黄酮合成基因表达量极少,这与茶树中类黄酮物质的分布规律一致。同时本研究在类黄酮调控相关MYB、bHLH、MADS、GST、WD40、Homeodomain基因家族中找到12个基因可能与花瓣中类黄酮物质合成调控有关,9个基因可能与芽中类黄酮物质合成调控有关。这些研究结果将为深入解析茶树类黄酮合成、调控机理打下基础。

关键词: 数字表达谱, 茶树, 类黄酮合成, 调控

Abstract: The expression of genes associated with flavonoid biosynthesis and its regulation in petal, pollen, dormant and sprouting buds of tea plants were analyzed by differential gene expression. The results showed that flavonoid biosynthetic genes were highly expressed in petal, dormant and sprouting buds, but rarely expressed in pollen, which is consistent with the distribution of flavonoids in tea plant. Furthermore, twelve genes in MYB, bHLH, MADS, GST, WD40 and Homeodomain gene families were identified to be correlated with flavonoid biosynthesis in petal. Nine genes were found to be involved in the regulation of flavonoid biosynthesis in tea buds. These results will be a basis for further understanding the mechanism of flavonoid biosynthetsis and regulation in Camellia sinensis.

Key words: digital gene expression profiling, tea plant, flavonoid biosynthesis, regulation

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