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茶叶科学 ›› 2015, Vol. 35 ›› Issue (6): 543-550.doi: 10.13305/j.cnki.jts.2015.06.005

• • 上一篇    下一篇

外源油菜素内酯对茶树光合特性的影响

李治鑫1,2, 李鑫1,*, 范利超1,2, 韩文炎1,*   

  1. 1. 中国农业科学院茶叶研究所,浙江 杭州310008;
    2. 中国农业科学院研究生院,北京 100081
  • 收稿日期:2015-06-01 修回日期:2015-09-09 出版日期:2015-12-15 发布日期:2019-08-26
  • 通讯作者: *hanwy@tricaas.com
  • 作者简介:李治鑫,男,硕士研究生,主要从事茶树栽培与生理生化研究。
  • 基金资助:
    中国农业科学院创新团队、国家自然科学基金(41171218)

Effects of Exogenous 24-epibrassinolide on the Photosynthetic Characteristics of Tea Plants (Camellia sinensis)

LI Zhixin1,2, LI Xin1,*, FAN Lichao1,2, HAN Wenyan1,*   

  1. 1. Tea Research Insititute, Chinese Academy of Agricultural Science, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-06-01 Revised:2015-09-09 Online:2015-12-15 Published:2019-08-26

摘要: 24-表油菜素内酯(EBR)是一种重要的植物激素,在植物对多种逆境的抵抗中发挥着重要的作用。本试验以田间龙井43、清明早和香菇寮白毫茶树品种为试验材料,研究了外源EBR处理对茶树叶片光合特性、Rubisco与FBPase活性及其相关基因表达量的影响。结果表明外源EBR(0.1βmg·L-1)处理后,龙井43、清明早和香菇寮白毫的叶片净光合速率分别提高了49.06%、45.49%和92.34%;最大羧化速率(Vcmax)分别提高了21.82%、21.68%和33.47%,最大再生速率(Jmax)分别提高了17.16%、23.86%和23.23%;Rubisco和FBPase活性显著提高(P<0.05);Rubisco和FBPase相关基因表达量显著(P<0.05)提高。可见,外源EBR可以通过调控基因表达提高茶树叶片中Rubisco和FBPase的活性以及Rubisco最大羧化速率和RuBP的最大再生速率,从而促进茶树光合碳固定,提高茶树的光合速率。

关键词: 茶树, 2-4表油菜素内酯, 光合特性, 基因表达

Abstract: 24-epibrassinolide (EBR) is an important plant hormone, which plays an important role in a variety of adverse resistance in plants. The effects of exogenous EBR on photosynthesis, Rubisco and FBPase activity and the expression of related-gene from tea leaves (Camellia sinensis cv. ‘Longjing 43’, ‘Xiangguliaobaihao’, and ‘Qingmingzao’) under field condition were investigated. It was showed that after exogenous EBR (0.1βmg·L-1) treatment: Net photosynthetic rate of these three cultivars increased by 49.06%, 45.49% and 92.34% respectively. Vcmax increased by 21.82%, 21.68 % and 21.68%, and Jmax increased by 17.16%, 23.86% and 23.86%, respectively. The activity of Rubisco and FBPase increased significantly. Furthermore, the expression of Rubisco and FBPase related-gene increased significantly. These results suggested that application of exogenous EBR could enhance Vcmax, Jmax and the activity of Rubisco and FBPase effectively, which ultimately promoted the photosynthetic carbon fixation, and the photosynthetic rate of tea plants.

Key words: tea plant (Camellia sinensis), 24-epibrassinolide, photosynthetic characteristics, gene expression

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