茶黄素对BSA/MDA羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究

doi:10.13305/j.cnki.jts.2016.04.004

茶叶科学 ›› 2016, Vol. 36 ›› Issue (4): 363-371.doi: 10.13305/j.cnki.jts.2016.04.004

茶黄素对BSA/MDA羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究

张静¹, 蔡淑娴², 黄建安^1,3,4, 李娟^1,3,4, 钟妮¹, 阳衡¹, 刘仲华^1,3,4,*

1. 湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2. 北京大学医学部,北京 100191;
3. 国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
4. 植物功能成分利用协同创新中心,湖南长沙 410128

收稿日期:2016-02-28 出版日期:2016-08-15 发布日期:2019-08-26
通讯作者: *,larkin-liu@163.com
作者简介:张静,女,博士,主要从事茶叶功能成分药理研究。
基金资助:
国家自然科学基金项目（31471590）、湖南省研究生科研创新项目（CX2015B245）、国家茶叶产业技术体系项目（CARS-23-11B）

Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro

ZHANG Jing¹, CAI Shuxian², HUANG Jian'an^1,3,4, LI Juan^1,3,4, ZHONG Ni¹, YANG Heng¹, LIU Zhonghua^1,3,4,*

1. Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, China;
2. Peking University Health Science Center, Beijing 100191, China;
3. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
4. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China

Received:2016-02-28 Online:2016-08-15 Published:2019-08-26

摘要/Abstract

摘要： 本实验构建了羰-氨交联反应形成老年色素的体外反应模型,通过ThT荧光、扫描电子显微镜、透射电镜、SDS-PAGE及NBT染色等检测手段,结果表明,茶黄素不仅可以抑制此模型中由于蛋白质羰基化产生的老年色素,还可以抑制此模型中因蛋白质聚集化产生的β-sheet结构。上述结果揭示了衰老机制中的蛋白质羰基化与导致神经退行性疾病的蛋白质聚集化之间存在一定的相关性。

关键词: 茶黄素, 羰-氨交联, 老年色素, 蛋白质聚集化

Abstract: A model of age pigment generation was builted by carbonyl-ammonia cross linking reaction in vitro, the datas indicate that theaflavins can not only inhibit the generation of age pigment in protein carbonylation, but also supress β-sheet-rich in aggregate protein in this model by thioflavin T-based fluorometric assay, scanning electron microscope, transmission electric microscope, SDS-PAGE and NBT staining, there are postive relationship between protein carbonylation on mechanism of aging and protein aggregation that lead to neurodegenerative disease.

Key words: theaflavin, carbonyl-ammonia cross linking reaction, age pigment, protein aggregation

中图分类号:

张静, 蔡淑娴, 黄建安, 李娟, 钟妮, 阳衡, 刘仲华. 茶黄素对BSA/MDA羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究[J]. 茶叶科学, 2016, 36(4): 363-371. doi: 10.13305/j.cnki.jts.2016.04.004.

ZHANG Jing, CAI Shuxian, HUANG Jian'an, LI Juan, ZHONG Ni, YANG Heng, LIU Zhonghua. Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro[J]. Journal of Tea Science, 2016, 36(4): 363-371. doi: 10.13305/j.cnki.jts.2016.04.004.

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茶黄素对BSA/MDA羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究

Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro

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